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Muscle-derived stem cell sheets support pump function and prevent cardiac arrhythmias in a model of chronic myocardial infarction

Sekiya, N and Tobita, K and Beckman, S and Okada, M and Gharaibeh, B and Sawa, Y and Kormos, RL and Huard, J (2013) Muscle-derived stem cell sheets support pump function and prevent cardiac arrhythmias in a model of chronic myocardial infarction. Molecular Therapy, 21 (3). 662 - 669. ISSN 1525-0016

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Abstract

Direct intracardiac cell injection for heart repair is hindered by numerous limitations including: cell death, poor spreading of the injected cells, arrhythmia, needle injury, etc. Tissue-engineered cell sheet implantation has the potential to overcome some of these limitations. We evaluated whether the transplantation of a muscle-derived stem cell (MDSC) sheet could improve the regenerative capacity of MDSCs in a chronic model of myocardial infarction. MDSC sheet-implanted mice displayed a reduction in left ventricle (LV) dilation and sustained LV contraction compared with the other groups. The MDSC sheet formed aligned myotubes and produced a significant increase in capillary density and a reduction of myocardial fibrosis compared with the other groups. Hearts transplanted with the MDSC sheets did not display any significant arrhythmias and the donor MDSC survival rate was higher than the direct myocardial MDSC injection group. MDSC sheet implantation yielded better functional recovery of chronic infarcted myocardium without any significant arrhythmic events compared with direct MDSC injection, suggesting this cell sheet delivery system could significantly improve the myocardial regenerative potential of the MDSCs. Copyright © 2013, The American Society of Gene & Cell Therapy.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Sekiya, N
Tobita, Kkit3@pitt.eduKIT3
Beckman, S
Okada, M
Gharaibeh, Bburhan@pitt.eduBURHAN0000-0002-5947-1232
Sawa, Y
Kormos, RLkormos@pitt.eduKORMOS
Huard, J
Centers: Other Centers, Institutes, or Units > McGowan Institute for Regenerative Medicine
Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 January 2013
Date Type: Publication
Journal or Publication Title: Molecular Therapy
Volume: 21
Number: 3
Page Range: 662 - 669
DOI or Unique Handle: 10.1038/mt.2012.266
Schools and Programs: School of Medicine > Cardiothoracic Surgery
School of Medicine > Developmental Biology
School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 1525-0016
MeSH Headings: Animals; Arrhythmias, Cardiac--physiopathology; Arrhythmias, Cardiac--prevention & control; Capillaries--physiopathology; Male; Mice; Mice, Inbred NOD; Muscle Cells--cytology; Myoblasts--cytology; Myoblasts--metabolism; Myoblasts--transplantation; Myocardial Infarction--physiopathology; Myocardial Infarction--therapy; Myocardium--cytology; Stem Cell Transplantation; Stem Cells--cytology; Tissue Engineering; Vascular Endothelial Growth Factor A--genetics; Vascular Endothelial Growth Factor A--metabolism; Ventricular Function, Left
Other ID: NLM PMC3589171
PubMed Central ID: PMC3589171
PubMed ID: 23319053
Date Deposited: 31 Mar 2014 15:19
Last Modified: 08 Jul 2018 00:55
URI: http://d-scholarship.pitt.edu/id/eprint/20832

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