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The use of blood vessel-derived stem cells for meniscal regeneration and repair

Osawa, A and Harner, CD and Gharaibeh, B and Matsumoto, T and Mifune, Y and Kopf, S and Ingham, SJM and Schreiber, V and Usas, A and Huard, J (2013) The use of blood vessel-derived stem cells for meniscal regeneration and repair. Medicine and Science in Sports and Exercise, 45 (5). 813 - 823. ISSN 0195-9131

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Abstract

Purpose: Surgical repairs of tears in the vascular region of the meniscus usually heal better than repairs performed in the avascular region; thus, we hypothesized that this region might possess a richer supply of vascular-derived stem cells than the avascular region. Methods: In this study, we analyzed 6 menisci extracted from aborted human fetuses and 12 human lateral menisci extracted from adult human subjects undergoing total knee arthroplasty. Menisci were immunostained for CD34 (a stem cell marker) and CD146 (a pericyte marker) in situ, whereas other menisci were dissected into two regions (peripheral and inner) and used to isolate meniscus-derived cells by flow cytometry. Cell populations expressing CD34 and CD146 were tested for their multilineage differentiation potentials, including chondrogenic, osteogenic, and adipogenic lineages. Fetal peripheral meniscus cells were transplanted by intracapsular injection into the knee joints of an athymic rat meniscal tear model. Rat menisci were extracted and histologically evaluated after 4 wk posttransplantation. Results: Immunohistochemistry and flow cytometric analyses demonstrated that a higher number of CD34- and CD146-positive cells were found in the peripheral region compared with the inner region. The CD34- and CD146-positive cells isolated from the vascular region of both fetal and adult menisci demonstrated multilineage differentiation capacities and were more potent than cells isolated from the inner (avascular) region. Fetal CD34- and CD146-positive cells transplanted into the athymic rat knee joint were recruited into the meniscal tear sites and contributed to meniscus repair. Conclusions: The vascularized region of the meniscus contains more stem cells than the avascular region. These meniscal-derived stem cells were multipotent and contributed to meniscal regeneration. Copyright © 2013 by the American College of Sports Medicine.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Osawa, A
Harner, CDcdh1@pitt.eduCDH1
Gharaibeh, Bburhan@pitt.eduBURHAN0000-0002-5947-1232
Matsumoto, T
Mifune, Y
Kopf, S
Ingham, SJM
Schreiber, V
Usas, A
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 May 2013
Date Type: Publication
Journal or Publication Title: Medicine and Science in Sports and Exercise
Volume: 45
Number: 5
Page Range: 813 - 823
DOI or Unique Handle: 10.1249/mss.0b013e31827d1e06
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 0195-9131
MeSH Headings: Aged; Animals; Antigens, CD146--metabolism; Antigens, CD34--metabolism; Disease Models, Animal; Female; Flow Cytometry; Humans; Immunohistochemistry; Male; Menisci, Tibial--cytology; Menisci, Tibial--surgery; Middle Aged; Rats; Regeneration--physiology; Wound Healing--physiology
PubMed ID: 23247715
Date Deposited: 31 Mar 2014 15:17
Last Modified: 23 May 2018 09:55
URI: http://d-scholarship.pitt.edu/id/eprint/20836

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