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Mechanical loading of stem cells for improvement of transplantation outcome in a model of acute myocardial infarction: The role of loading history

UNSPECIFIED (2012) Mechanical loading of stem cells for improvement of transplantation outcome in a model of acute myocardial infarction: The role of loading history. Tissue Engineering - Part A, 18 (11-12). 1101 - 1108. ISSN 1937-3341

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Abstract

Stem cell therapy for tissue repair is a rapidly evolving field and the factors that dictate the physiological responsiveness of stem cells remain under intense investigation. In this study we hypothesized that the mechanical loading history of muscle-derived stem cells (MDSCs) would significantly impact MDSC survival, host tissue angiogenesis, and myocardial function after MDSC transplantation into acutely infarcted myocardium. Mice with acute myocardial infarction by permanent left coronary artery ligation were injected with either nonstimulated (NS) or mechanically stimulated (MS) MDSCs. Mechanical stimulation consisted of stretching the cells with equibiaxial stretch with a magnitude of 10% and frequency of 0.5Hz. MS cell-transplanted hearts showed improved cardiac contractility, increased numbers of host CD31+ cells, and decreased fibrosis, in the peri-infarct region, compared to the hearts treated with NS MDSCs. MS MDSCs displayed higher vascular endothelial growth factor expression than NS cells in vitro. These findings highlight an important role for cyclic mechanical loading preconditioning of donor MDSCs in optimizing MDSC transplantation for myocardial repair. © 2012, Mary Ann Liebert, Inc.


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Details

Item Type: Article
Status: Published
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 June 2012
Date Type: Publication
Journal or Publication Title: Tissue Engineering - Part A
Volume: 18
Number: 11-12
Page Range: 1101 - 1108
DOI or Unique Handle: 10.1089/ten.tea.2011.0285
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Medicine > Pathology
School of Medicine > Pediatrics
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 1937-3341
Article Type: Review
MeSH Headings: Animals; Biomechanical Phenomena; Cell Differentiation; Cell Proliferation; Cell Survival; Cicatrix--pathology; Cicatrix--physiopathology; Disease Models, Animal; Heart Function Tests; Ischemic Preconditioning, Myocardial; Male; Mice; Mice, Inbred C57BL; Myocardial Infarction--pathology; Myocardial Infarction--physiopathology; Myocardial Infarction--therapy; Myocardium--pathology; Neovascularization, Physiologic; Oxidative Stress; Stem Cell Transplantation; Stem Cells--cytology; Stress, Mechanical; Treatment Outcome; Vascular Endothelial Growth Factor A--metabolism
Other ID: NLM PMC3360502
PubMed Central ID: PMC3360502
PubMed ID: 22280442
Date Deposited: 04 Apr 2014 15:45
Last Modified: 06 Jan 2019 01:55
URI: http://d-scholarship.pitt.edu/id/eprint/20840

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