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Role of angiogenesis after muscle derived stem cell transplantation in injured medial collateral ligament

Nishimori, M and Matsumoto, T and Ota, S and Kopf, S and Mifune, Y and Harner, C and Ochi, M and Fu, FH and Huard, J (2012) Role of angiogenesis after muscle derived stem cell transplantation in injured medial collateral ligament. Journal of Orthopaedic Research, 30 (4). 627 - 633. ISSN 0736-0266

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We performed this study to investigate the therapeutic role of vascular endothelial growth factor (VEGF) in medial collateral ligament (MCL) healing. Murine muscle derived stem cells (MDSCs) obtained via the preplate technique were retrovirally transduced to express: (1) VEGF and nLacZ (MDSC-VEGF), (2) soluble fms-like tyrosine kinase-1 (sFLT1, a VEGF-specific antagonist) and nLacZ (MDSC-sFLT1), and (3) nLacZ (MDSC-nLacZ). After transecting the MCL of immunodeficient rats, 5 × 10 5 cells of each of the transduction groups list above were transplanted into the MCL injury site. A control group was injected with phosphate-buffered saline (PBS) only. Immunohistochemical staining demonstrated that there were more Isolectin B4 and β-galactosidase double positive cells in the rats transplanted with MDSC-VEGF transduced cells than the other groups at week 1. Capillary density was significantly higher in the MDSC-VEGF group than the other groups at week 2; however, there were no significant differences in the biomechanical assessment between the MDSC-VEGF and MDSC-nLacZ groups. On the other hand, the MDSC-sFLT1 group revealed a lower capillary density than the other two groups and the functional ligament healing of the MDSC-sFLT1 group was significantly decreased compared to the other groups when assessed biomechanically. The findings of the present study suggest that angiogenesis plays a critical role in the healing process of injured MCL. Copyright © 2011 Orthopaedic Research Society.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Nishimori, M
Matsumoto, T
Ota, S
Kopf, S
Mifune, Y
Harner, Ccdh1@pitt.eduCDH1
Ochi, M
Fu, FHffu@pitt.eduFFU
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 April 2012
Date Type: Publication
Journal or Publication Title: Journal of Orthopaedic Research
Volume: 30
Number: 4
Page Range: 627 - 633
DOI or Unique Handle: 10.1002/jor.21551
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 0736-0266
MeSH Headings: Animals; Biomechanical Phenomena--physiology; Cells, Cultured; Female; Knee Injuries--physiopathology; Knee Injuries--therapy; Lac Operon; Medial Collateral Ligament, Knee--blood supply; Medial Collateral Ligament, Knee--cytology; Medial Collateral Ligament, Knee--injuries; Mice; Mice, Inbred C57BL; Muscle, Skeletal--cytology; Neovascularization, Physiologic--physiology; Rats; Rats, Nude; Recovery of Function--physiology; Stem Cell Transplantation--methods; Stem Cells--cytology; Vascular Endothelial Growth Factor A--genetics; Vascular Endothelial Growth Factor A--physiology; Vascular Endothelial Growth Factor Receptor-1--metabolism; Wound Healing--physiology
PubMed ID: 21913220
Date Deposited: 04 Apr 2014 15:41
Last Modified: 22 Jun 2021 14:55


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