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Use of an ultrasonic blade facilitates muscle repair after incision injury

Usas, A and Usaite, D and Gao, X and Huard, J and Clymer, JW and Malaviya, P (2011) Use of an ultrasonic blade facilitates muscle repair after incision injury. Journal of Surgical Research, 167 (2). ISSN 0022-4804

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Abstract

Background: The ultrasonic Harmonic Blade cuts and coagulates soft tissue at temperatures lower than conventional electrosurgery. This study investigated whether improved hemostatic control and reduced collateral damage in skeletal muscle incisions translates into improved myofiber regeneration, reduced fibrosis and faster muscle recovery. Materials and Methods: Transections in the left gastrocnemius muscles of mice were made with the Harmonic Blade, and contralaterally, with either cold steel scissors or electrosurgery. Histology up to 8 wk after surgery was performed to evaluate myofiber regeneration and fibrosis. Tissue inflammation (Gr1+ neutrophils) and vascularization (CD31+ capillaries) were assessed immunohistochemically at 1 wk . Results: Overall the Harmonic Blade showed significantly higher level of muscle regeneration than cold steel. Fibrosis for both the Harmonic Blade and cold steel decreased three-fold over the 8 wk period, while electrosurgery yielded significantly increasing fibrosis through wk 4 before declining. At 1 wk post-surgery the Harmonic Blade induced less inflammation than electrosurgery, and higher vascularization than electrosurgery and cold steel. Conclusions: Harmonic Blade-incised tissue showed accelerated vascularization, slight reduction of inflammation, enhanced muscle regeneration and decreased scarring, demonstrating a more effective healing process than electrosurgery. © 2011 Elsevier Inc. All rights reserved.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Usas, A
Usaite, D
Gao, X
Huard, J
Clymer, JW
Malaviya, P
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 15 May 2011
Date Type: Publication
Journal or Publication Title: Journal of Surgical Research
Volume: 167
Number: 2
DOI or Unique Handle: 10.1016/j.jss.2010.12.042
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 0022-4804
MeSH Headings: Animals; Blood Vessels--pathology; Electrosurgery; Female; Fibrosis; Mice; Mice, Inbred C57BL; Models, Animal; Muscle, Skeletal--pathology; Muscle, Skeletal--physiology; Muscle, Skeletal--surgery; Regeneration--physiology; Ultrasonics; Wound Healing--physiology
PubMed ID: 21324491
Date Deposited: 04 Apr 2014 15:57
Last Modified: 29 Jan 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/20888

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