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Placental perivascular cells for human muscle regeneration

Park, TS and Gavina, M and Chen, CW and Sun, B and Teng, PN and Huard, J and Deasy, BM and Zimmerlin, L and Péault, B (2011) Placental perivascular cells for human muscle regeneration. Stem Cells and Development, 20 (3). 451 - 463. ISSN 1547-3287

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Perivascular multipotent mesenchymal progenitors exist in a variety of tissues, including the placenta. Here, we suggest that the abundant vasculature present in the human placenta can serve as a source of myogenic cells to regenerate skeletal muscle. Chorionic villi dissected from the mid-gestation human placenta were first transplanted intact into the gastrocnemius muscles of SCID/mdx mice, where they participated in muscle regeneration by producing myofibers expressing human dystrophin and spectrin. In vitro-cultured placental villi released rapidly adhering and migratory CD146+CD34-CD45-CD56- cells of putative perivascular origin that expressed mesenchymal stem cell markers. CD146+CD34-CD45-CD56- perivascular cells isolated and purified from the placental villi by flow cytometry were indeed highly myogenic in culture, and generated dystrophin-positive myofibers, and they promoted angiogenesis after transplantation into SCID/mdx mouse muscles. These observations confirm the existence of mesenchymal progenitor cells within the walls of human blood vessels, and suggest that the richly vascularized human placenta is an abundant source of perivascular myogenic cells able to migrate within dystrophic muscle and regenerate myofibers. © 2011, Mary Ann Liebert, Inc.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Park, TS
Gavina, M
Chen, CW
Sun, Bbis3@pitt.eduBIS3
Teng, PN
Huard, J
Deasy, BM
Zimmerlin, L
Péault, B
Centers: Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine
Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 March 2011
Date Type: Publication
Journal or Publication Title: Stem Cells and Development
Volume: 20
Number: 3
Page Range: 451 - 463
DOI or Unique Handle: 10.1089/scd.2010.0354
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 1547-3287
MeSH Headings: Animals; Antigens, CD--genetics; Antigens, CD--metabolism; Antigens, Nuclear--metabolism; Cell Adhesion; Cell Differentiation; Cell Movement; Cell Shape; Cells, Cultured; Chorionic Villi--metabolism; Chorionic Villi--transplantation; Dystrophin--metabolism; Female; Humans; Mesenchymal Stromal Cells--metabolism; Mice; Mice, SCID; Muscle Fibers, Skeletal--cytology; Muscle Fibers, Skeletal--metabolism; Muscle, Skeletal--blood supply; Muscle, Skeletal--cytology; Muscle, Skeletal--physiology; Neovascularization, Physiologic; Placenta--blood supply; Placenta--cytology; Pregnancy; Regeneration; Spectrin--metabolism; Tissue Culture Techniques; Transcription, Genetic
Other ID: NLM PMC3120979
PubMed Central ID: PMC3120979
PubMed ID: 20923371
Date Deposited: 04 Apr 2014 15:56
Last Modified: 29 Jan 2019 15:55


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