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Inkjet-based biopatterning of bone morphogenetic protein-2 to spatially control calvarial bone formation

Cooper, GM and Miller, ED and Decesare, GE and Usas, A and Lensie, EL and Bykowski, MR and Huard, J and Weiss, LE and Losee, JE and Campbell, PG (2010) Inkjet-based biopatterning of bone morphogenetic protein-2 to spatially control calvarial bone formation. Tissue Engineering - Part A, 16 (5). 1749 - 1759. ISSN 1937-3341

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Abstract

The purpose of this study was to demonstrate spatial control of osteoblast differentiation in vitro and bone formation in vivo using inkjet bioprinting technology and to create three-dimensional persistent bio-ink patterns of bone morphogenetic protein-2 (BMP-2) and its modifiers immobilized within microporous scaffolds. Semicircular patterns of BMP-2 were printed within circular DermaMatrix™ human allograft scaffold constructs. The contralateral halves of the constructs were unprinted or printed with BMP-2 modifiers, including the BMP-2 inhibitor, noggin. Printed bio-ink pattern retention was validated using fluorescent or 125I-labeled bio-inks. Mouse C2C12 progenitor cells cultured on patterned constructs differentiated in a dose-dependent fashion toward an osteoblastic fate in register to BMP-2 patterns. The fidelity of spatial restriction of osteoblastic differentiation at the boundary between neighboring BMP-2 and noggin patterns improved in comparison with patterns without noggin. Acellular DermaMatrix constructs similarly patterned with BMP-2 and noggin were then implanted into a mouse calvarial defect model. Patterns of bone formation in vivo were comparable with patterned responses of osteoblastic differentiation in vitro. These results demonstrate that three-dimensional biopatterning of a growth factor and growth factor modifier within a construct can direct cell differentiation in vitro and tissue formation in vivo in register to printed patterns. © 2010 Mary Ann Liebert, Inc.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Cooper, GMgmc8@pitt.eduGMC8
Miller, ED
Decesare, GE
Usas, A
Lensie, EL
Bykowski, MR
Huard, J
Weiss, LE
Losee, JEjel35@pitt.eduJEL35
Campbell, PG
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 May 2010
Date Type: Publication
Journal or Publication Title: Tissue Engineering - Part A
Volume: 16
Number: 5
Page Range: 1749 - 1759
DOI or Unique Handle: 10.1089/ten.tea.2009.0650
Schools and Programs: School of Medicine > Surgery
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 1937-3341
MeSH Headings: Animals; Bone Morphogenetic Protein 2--pharmacology; Cell Differentiation--drug effects; Humans; Male; Mice; Mice, Inbred C57BL; Microscopy, Electron, Scanning; Osteogenesis--drug effects; Skull--drug effects; Skull--growth & development; Tissue Engineering--instrumentation; Tissue Engineering--methods; Tissue Scaffolds--chemistry
Other ID: NLM PMC2952127
PubMed Central ID: PMC2952127
PubMed ID: 20028232
Date Deposited: 04 Apr 2014 16:27
Last Modified: 23 Feb 2019 16:55
URI: http://d-scholarship.pitt.edu/id/eprint/20931

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