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In Vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model

Nieponice, A and Soletti, L and Guan, J and Hong, Y and Gharaibeh, B and Maul, TM and Huard, J and Wagner, WR and Vorp, DA (2010) In Vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model. Tissue Engineering - Part A, 16 (4). 1215 - 1223. ISSN 1937-3341

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Limited autologous vascular graft availability and poor patency rates of synthetic grafts for bypass or replacement of small-diameter arteries remain a concern in the surgical community. These limitations could potentially be improved by a tissue engineering approach. We report here our progress in the development and in vivo testing of a stem-cell-based tissue-engineered vascular graft for arterial applications. Poly(ester urethane)urea scaffolds (length=10mm; inner diameter=1.2mm) were created by thermally induced phase separation (TIPS). Compound scaffolds were generated by reinforcing TIPS scaffolds with an outer electrospun layer of the same biomaterial (ES-TIPS). Both TIPS and ES-TIPS scaffolds were bulk-seeded with 10×106 allogeneic, LacZ-transfected, muscle-derived stem cells (MDSCs), and then placed in spinner flask culture for 48h. Constructs were implanted as interposition grafts in the abdominal aorta of rats for 8 weeks. Angiograms and histological assessment were performed at the time of explant. Cell-seeded constructs showed a higher patency rate than the unseeded controls: 65% (ES-TIPS) and 53% (TIPS) versus 10% (acellular TIPS). TIPS scaffolds had a 50% mechanical failure rate with aneurysmal formation, whereas no dilation was observed in the hybrid scaffolds. A smooth-muscle-like layer of cells was observed near the luminal surface of the constructs that stained positive for smooth muscle α-actin and calponin. LacZ+ cells were shown to be engrafted in the remodeled construct. A confluent layer of von Willebrand Factor-positive cells was observed in the lumen of MDSC-seeded constructs, whereas acellular controls showed platelet and fibrin deposition. This is the first evidence that MDSCs improve patency and contribute to the remodeling of a tissue-engineered vascular graft for arterial applications. © 2010 Mary Ann Liebert, Inc.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Nieponice, A
Soletti, L
Guan, J
Hong, Y
Gharaibeh, Bburhan@pitt.eduBURHAN0000-0002-5947-1232
Maul, TM
Huard, J
Wagner, WRwagner@pitt.eduWAGNER0000-0003-0082-8089
Vorp, DAvorp@pitt.eduVORP0000-0002-6467-2151
Centers: Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine
Date: 1 April 2010
Date Type: Publication
Journal or Publication Title: Tissue Engineering - Part A
Volume: 16
Number: 4
Page Range: 1215 - 1223
DOI or Unique Handle: 10.1089/ten.tea.2009.0427
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Medicine > Surgery
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 1937-3341
MeSH Headings: Adult Stem Cells--cytology; Adult Stem Cells--metabolism; Adult Stem Cells--transplantation; Animals; Aorta, Abdominal--surgery; Biocompatible Materials; Blood Vessel Prosthesis; Elastomers; Lac Operon; Microscopy, Electron, Scanning; Myocytes, Smooth Muscle--cytology; Myocytes, Smooth Muscle--metabolism; Myocytes, Smooth Muscle--transplantation; Polyesters; Rats; Rats, Inbred Lew; Tissue Engineering--methods; Tissue Scaffolds--chemistry; Transfection; Transplantation, Homologous
Other ID: NLM PMC2862609
PubMed Central ID: PMC2862609
PubMed ID: 19895206
Date Deposited: 04 Apr 2014 16:26
Last Modified: 19 Mar 2024 19:55


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