Nieponice, A and Soletti, L and Guan, J and Hong, Y and Gharaibeh, B and Maul, TM and Huard, J and Wagner, WR and Vorp, DA
(2010)
In Vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model.
Tissue Engineering - Part A, 16 (4).
1215 - 1223.
ISSN 1937-3341
Abstract
Limited autologous vascular graft availability and poor patency rates of synthetic grafts for bypass or replacement of small-diameter arteries remain a concern in the surgical community. These limitations could potentially be improved by a tissue engineering approach. We report here our progress in the development and in vivo testing of a stem-cell-based tissue-engineered vascular graft for arterial applications. Poly(ester urethane)urea scaffolds (length=10mm; inner diameter=1.2mm) were created by thermally induced phase separation (TIPS). Compound scaffolds were generated by reinforcing TIPS scaffolds with an outer electrospun layer of the same biomaterial (ES-TIPS). Both TIPS and ES-TIPS scaffolds were bulk-seeded with 10×106 allogeneic, LacZ-transfected, muscle-derived stem cells (MDSCs), and then placed in spinner flask culture for 48h. Constructs were implanted as interposition grafts in the abdominal aorta of rats for 8 weeks. Angiograms and histological assessment were performed at the time of explant. Cell-seeded constructs showed a higher patency rate than the unseeded controls: 65% (ES-TIPS) and 53% (TIPS) versus 10% (acellular TIPS). TIPS scaffolds had a 50% mechanical failure rate with aneurysmal formation, whereas no dilation was observed in the hybrid scaffolds. A smooth-muscle-like layer of cells was observed near the luminal surface of the constructs that stained positive for smooth muscle α-actin and calponin. LacZ+ cells were shown to be engrafted in the remodeled construct. A confluent layer of von Willebrand Factor-positive cells was observed in the lumen of MDSC-seeded constructs, whereas acellular controls showed platelet and fibrin deposition. This is the first evidence that MDSCs improve patency and contribute to the remodeling of a tissue-engineered vascular graft for arterial applications. © 2010 Mary Ann Liebert, Inc.
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
|
| Centers: |
Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine |
| Date: |
1 April 2010 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Tissue Engineering - Part A |
| Volume: |
16 |
| Number: |
4 |
| Page Range: |
1215 - 1223 |
| DOI or Unique Handle: |
10.1089/ten.tea.2009.0427 |
| Schools and Programs: |
School of Medicine > Orthopaedic Surgery
School of Medicine > Surgery
Swanson School of Engineering > Bioengineering |
| Refereed: |
Yes |
| ISSN: |
1937-3341 |
| MeSH Headings: |
Adult Stem Cells--cytology; Adult Stem Cells--metabolism; Adult Stem Cells--transplantation; Animals; Aorta, Abdominal--surgery; Biocompatible Materials; Blood Vessel Prosthesis; Elastomers; Lac Operon; Microscopy, Electron, Scanning; Myocytes, Smooth Muscle--cytology; Myocytes, Smooth Muscle--metabolism; Myocytes, Smooth Muscle--transplantation; Polyesters; Rats; Rats, Inbred Lew; Tissue Engineering--methods; Tissue Scaffolds--chemistry; Transfection; Transplantation, Homologous |
| Other ID: |
NLM PMC2862609 |
| PubMed Central ID: |
PMC2862609 |
| PubMed ID: |
19895206 |
| Date Deposited: |
04 Apr 2014 16:26 |
| Last Modified: |
19 Mar 2024 19:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/20932 |
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