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Skeletal muscle-derived stem cells differentiate into hepatocyte-like cells and aid in liver regeneration

Bellayr, IH and Gharaibeh, B and Huard, J and Li, Y (2010) Skeletal muscle-derived stem cells differentiate into hepatocyte-like cells and aid in liver regeneration. International Journal of Clinical and Experimental Pathology, 3 (7). 681 - 690.

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Abstract

The liver is unique for its ability to regenerate after injury, however, critical injuries or disease cause it to lose this quality. Stem cells have been explored as a possibility to restore the function of seriously damaged livers, based on their self-renewability and multiple differentiation capacity. These experiments examine the ability of muscle derived stem cells (MDSCs) to differentiate into hepatocyte-like cells in vitro and acquire functional liver attributes for repairing damaged livers. In vitro experiments were performed using MDSCs from postnatal mice and mouse hepatocyte cell lines. Our data revealed that MDSCs differentiated into hepatocyte-like cells and expressed liver cell markers, albumin, hepatocyte nuclear factor 4α, and alpha feto-protein, both at the RNA and protein level. Additionally, in vivo studies showed successful engraftment of MDSCs into hepatectomized mouse livers of mice. These results provide evidence suggesting that MDSCs have the capacity to differentiate into liver cell-like cells and may serve as potential candidates to aid in liver regeneration.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Bellayr, IH
Gharaibeh, Bburhan@pitt.eduBURHAN0000-0002-5947-1232
Huard, J
Li, Y
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 September 2010
Date Type: Publication
Journal or Publication Title: International Journal of Clinical and Experimental Pathology
Volume: 3
Number: 7
Page Range: 681 - 690
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Medicine > Pathology
Swanson School of Engineering > Bioengineering
Refereed: Yes
Related URLs:
MeSH Headings: Animals; Cell Differentiation--physiology; Coculture Techniques; Hepatocytes--cytology; Immunohistochemistry; In Situ Hybridization, Fluorescence; Liver Regeneration--physiology; Mice; Muscle, Skeletal--cytology; Reverse Transcriptase Polymerase Chain Reaction; Stem Cells--cytology
Other ID: NLM PMC2933388
PubMed Central ID: PMC2933388
PubMed ID: 20830239
Date Deposited: 04 Apr 2014 16:44
Last Modified: 25 Jan 2019 23:55
URI: http://d-scholarship.pitt.edu/id/eprint/20944

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