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Pericyte-based human tissue engineered vascular grafts

He, W and Nieponice, A and Soletti, L and Hong, Y and Gharaibeh, B and Crisan, M and Usas, A and Peault, B and Huard, J and Wagner, WR and Vorp, DA (2010) Pericyte-based human tissue engineered vascular grafts. Biomaterials, 31 (32). 8235 - 8244. ISSN 0142-9612

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Abstract

The success of small-diameter tissue engineered vascular grafts (TEVGs) greatly relies on an appropriate cell source and an efficient cellular delivery and carrier system. Pericytes have recently been shown to express mesenchymal stem cell features. Their relative availability and multipotentiality make them a promising candidate for TEVG applications. The objective of this study was to incorporate pericytes into a biodegradable scaffold rapidly, densely and efficiently, and to assess the efficacy of the pericyte-seeded scaffold in vivo. Bi-layered elastomeric poly(ester-urethane)urea scaffolds (length = 10 mm; inner diameter = 1.3 mm) were bulk seeded with 3 × 106 pericytes using a customized rotational vacuum seeding device in less than 2 min (seeding efficiency > 90%). The seeded scaffolds were cultured in spinner flasks for 2 days and then implanted into Lewis rats as aortic interposition grafts for 8 weeks. Results showed pericytes populated the porous layer of the scaffolds evenly and maintained their original phenotype after the dynamic culture. After implantation, pericyte-seeded TEVGs showed a significant higher patency rate than the unseeded control: 100% versus 38% (p < 0.05). Patent pericyte-seeded TEVGs revealed extensive tissue remodeling with collagen and elastin present. The remodeled tissue consisted of multiple layers of α-smooth muscle actin- and calponin-positive cells, and a von Willebrand factor-positive monolayer in the lumen. These results demonstrate the feasibility of a pericyte-based TEVG and suggest that the pericytes play a role in maintaining patency of the TEVG as an arterial conduit. © 2010 Elsevier Ltd.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
He, W
Nieponice, A
Soletti, L
Hong, Y
Gharaibeh, Bburhan@pitt.eduBURHAN0000-0002-5947-1232
Crisan, M
Usas, A
Peault, B
Huard, J
Wagner, WRwagner@pitt.eduWAGNER
Vorp, DAvorp@pitt.eduVORP0000-0002-6467-2151
Centers: Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine
Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 November 2010
Date Type: Publication
Journal or Publication Title: Biomaterials
Volume: 31
Number: 32
Page Range: 8235 - 8244
DOI or Unique Handle: 10.1016/j.biomaterials.2010.07.034
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Medicine > Pediatrics
School of Medicine > Surgery
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 0142-9612
MeSH Headings: Animals; Aorta--ultrastructure; Blood Vessel Prosthesis; Blood Vessel Prosthesis Implantation; Cells, Cultured; Female; Humans; Pericytes--cytology; Rats; Rats, Inbred Lew; Tissue Engineering--methods; Tissue Scaffolds--chemistry; Vascular Patency
Other ID: NLM NIHMS222782, NLM PMC3178347
PubMed Central ID: PMC3178347
PubMed ID: 20684982
Date Deposited: 04 Apr 2014 16:41
Last Modified: 02 Dec 2021 18:55
URI: http://d-scholarship.pitt.edu/id/eprint/20946

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