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Antioxidant levels represent a major determinant in the regenerative capacity of muscle stem cells

Urish, KL and Vella, JB and Okada, M and Deasy, BM and Tobita, K and Keller, BB and Cao, B and Piganelli, JD and Huard, J (2009) Antioxidant levels represent a major determinant in the regenerative capacity of muscle stem cells. Molecular Biology of the Cell, 20 (1). 509 - 520. ISSN 1059-1524

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Abstract

Stem cells are classically defined by their multipotent, long-term proliferation, and self-renewal capabilities. Here, we show that increased antioxidant capacity represents an additional functional characteristic of muscle-derived stem cells (MDSCs). Seeking to understand the superior regenerative capacity of MDSCs compared with myoblasts in cardiac and skeletal muscle transplantation, our group hypothesized that survival of the oxidative and inflammatory stress inherent to transplantation may play an important role. Evidence of increased enzymatic and nonenzymatic antioxidant capacity of MDSCs were observed in terms of higher levels of superoxide dismutase and glutathione, which appears to confer a differentiation and survival advantage. Further when glutathione levels of the MDSCs are lowered to that of myoblasts, the transplantation advantage of MDSCs over myoblasts is lost when transplanted into both skeletal and cardiac muscles. These findings elucidate an important cause for the superior regenerative capacity of MDSCs, and provide functional evidence for the emerging role of antioxidant capacity as a critical property for MDSC survival post-transplantation.© 2008 by The American Society for Cell Biology.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Urish, KL
Vella, JB
Okada, M
Deasy, BM
Tobita, Kkit3@pitt.eduKIT3
Keller, BB
Cao, B
Piganelli, JDjdp51@pitt.eduJDP51
Huard, J
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorGutkind, J SilvioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 January 2009
Date Type: Publication
Journal or Publication Title: Molecular Biology of the Cell
Volume: 20
Number: 1
Page Range: 509 - 520
DOI or Unique Handle: 10.1091/mbc.e08-03-0274
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
School of Medicine > Pediatrics
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 1059-1524
MeSH Headings: Animals; Antioxidants--metabolism; Cell Death--physiology; Cell Differentiation--physiology; Hydrogen Peroxide--metabolism; Mice; Mice, Inbred C57BL; Muscle, Skeletal--cytology; Muscle, Skeletal--physiology; Myoblasts--cytology; Myoblasts--physiology; Myocytes, Cardiac--cytology; Myocytes, Cardiac--physiology; Oxidants--metabolism; Oxidative Stress; Reactive Oxygen Species--metabolism; Regeneration--physiology; Stem Cells--cytology; Stem Cells--physiology; Tumor Necrosis Factor-alpha--metabolism
Other ID: NLM PMC2613087
PubMed Central ID: PMC2613087
PubMed ID: 19005220
Date Deposited: 09 Apr 2014 16:43
Last Modified: 03 Feb 2019 01:55
URI: http://d-scholarship.pitt.edu/id/eprint/21043

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