Matsumoto, T and Cooper, GM and Gharaibeh, B and Meszaros, LB and Li, G and Usas, A and Fu, FH and Huard, J
(2009)
Cartilage repair in a rat model of osteoarthritis through intraarticular transplantation of muscle-derived stem cells expressing bone morphogenetic protein 4 and soluble Flt-1.
Arthritis and Rheumatism, 60 (5).
1390 - 1405.
ISSN 0004-3591
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Abstract
Objective. The control of angiogenesis during chondrogenic differentiation is an important issue affecting the use of stem cells in cartilage repair, especially with regard to the persistence of regenerated cartilage. This study was undertaken to investigate the effect of vascular endothelial growth factor (VEGF) stimulation and the blocking of VEGF with its antagonist, soluble Flt-1 (sFlt-1), on the chondrogenesis of skeletal muscle-derived stem cells (MDSCs) in a rat model of osteoarthritis (OA). Methods. We investigated the effect of VEGF on cartilage repair in an immunodeficiency rat model of OA after intraarticular injection of murine MDSCs expressing bone morphogenetic protein 4 (BMP-4) in combination with MDSCs expressing VEGF or sFlt-1. Results. In vivo, a combination of sFlt-1- and BMP-4-transduced MDSCs demonstrated better repair without osteophyte formation macroscopically and histologically following OA induction, when compared with the other groups. Higher differentiation/ proliferation and lower levels of chondrocyte apoptosis were also observed in sFlt-1- and BMP-4-transduced MDSCs compared with a combination of VEGF- and BMP-4-transduced MDSCs or with BMP-4-transduced MDSCs alone. In vitro experiments with mixed pellet coculture of MDSCs and OA chondrocytes revealed that BMP-4-transduced MDSCs produced the largest pellets, which had the highest gene expression of not only type II collagen and SOX9 but also type X collagen, suggesting formation of hypertrophic chondrocytes. Conclusion. Our results demonstrate that MDSC-based therapy involving sFlt-1 and BMP-4 repairs articular cartilage in OA mainly by having a beneficial effect on chondrogenesis by the donor and host cells as well as by preventing angiogenesis, which eventually prevents cartilage resorption, resulting in persistent cartilage regeneration and repair. © 2009, American College of Rheumatology.
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
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| Date: |
1 May 2009 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Arthritis and Rheumatism |
| Volume: |
60 |
| Number: |
5 |
| Page Range: |
1390 - 1405 |
| DOI or Unique Handle: |
10.1002/art.24443 |
| Schools and Programs: |
School of Medicine > Pediatrics |
| Refereed: |
Yes |
| ISSN: |
0004-3591 |
| MeSH Headings: |
Animals; Bone Morphogenetic Protein 4--analysis; Bone Morphogenetic Protein 4--pharmacology; Cartilage, Articular--physiology; Chondrogenesis--physiology; Female; Male; Mice; Mice, Inbred C57BL; Neovascularization, Pathologic--prevention & control; Osteoarthritis--surgery; Rats; Rats, Nude; Regeneration--physiology; Stem Cell Transplantation; Transduction, Genetic; Vascular Endothelial Growth Factor Receptor-1--analysis; Vascular Endothelial Growth Factor Receptor-1--physiology |
| PubMed ID: |
19404941 |
| Date Deposited: |
09 Apr 2014 16:33 |
| Last Modified: |
04 Feb 2019 15:56 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/21049 |
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