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Sex of muscle stem cells does not influence potency for cardiac cell therapy

Drowley, L and Okada, M and Payne, TR and Botta, GP and Oshima, H and Keller, BB and Tobita, K and Huard, J (2009) Sex of muscle stem cells does not influence potency for cardiac cell therapy. Cell Transplantation, 18 (10-11). 1137 - 1146. ISSN 0963-6897

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We have previously shown that populations of skeletal muscle-derived stem cells (MDSCs) exhibit sexbased differences for skeletal muscle and bone repair, with female cells demonstrating superior engrafting abilities to males in skeletal muscle while male cells differentiating more robustly toward the osteogenic and chondrogenic lineages. In this study, we tested the hypothesis that the therapeutic capacity of MDSCs transplanted into myocardium is influenced by sex of donor MDSCs or recipient. Male and female MDSCs isolated from the skeletal muscle of 3-week-old mice were transplanted into recipient male or female dystrophin-deficient (mdx) hearts or into the hearts of male SCID mice following acute myocardial infarction. In the mdx model, no difference was seen in engraftment or blood vessel formation based on donor cell or recipient sex. In the infarction model, MDSC-transplanted hearts showed higher postinfarction angiogenesis, less myocardial scar formation, and improved cardiac function compared to vehicle controls. However, sex of donor MDSCs had no significant effects on engraftment, angiogenesis, and cardiac function. VEGF expression, a potent angiogenic factor, was similar between male and female MDSCs. Our results suggest that donor MDSC or recipient sex has no significant effect on the efficiency of MDSC-triggered myocardial engraftment or regeneration following cardiac injury. The ability of the MDSCs to improve cardiac regeneration and repair through promotion of angiogenesis without differentiation into the cardiac lineage may have contributed to the lack of sex difference observed in these models. Copyright © 2009 Cognizant Comm. Corp.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Drowley, L
Okada, M
Payne, TR
Botta, GP
Oshima, H
Keller, BB
Tobita, Kkit3@pitt.eduKIT3
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 December 2009
Date Type: Publication
Journal or Publication Title: Cell Transplantation
Volume: 18
Number: 10-11
Page Range: 1137 - 1146
DOI or Unique Handle: 10.3727/096368909x471305
Schools and Programs: School of Medicine > Developmental Biology
School of Medicine > Orthopaedic Surgery
School of Medicine > Pathology
Refereed: Yes
ISSN: 0963-6897
MeSH Headings: Animals; Cell Lineage; Dystrophin--deficiency; Dystrophin--genetics; Dystrophin--metabolism; Female; Heart--physiology; Male; Mice; Mice, Inbred C57BL; Mice, SCID; Muscle, Skeletal--cytology; Myocardial Infarction--therapy; Myocardium--pathology; Sex Factors; Stem Cell Transplantation; Vascular Endothelial Growth Factor A--metabolism
PubMed ID: 19523348
Date Deposited: 09 Apr 2014 16:31
Last Modified: 03 Feb 2019 01:55


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