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Development of a Deep UV Resonance Raman Spectrometer

Wert, Jonathan D. (2014) Development of a Deep UV Resonance Raman Spectrometer. Master's Thesis, University of Pittsburgh. (Unpublished)

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UV resonance Raman spectroscopy is well-suited for studies of protein structure and dynamics due to its high sensitivity and selectivity. This has allowed for equilibrium as well as kinetic studies of protein folding, investigations of DNA-protein interactions, and characterization of explosive species.
This thesis is focused on a plan for the development of a deep UV resonance Raman spectrometer for excitation wavelengths from 197 nm – 240 nm. This spectrometer will utilize two gratings in a subtractive dispersion mode, giving a theoretical reciprocal linear dispersion of 0.424 nm/mm. This gives a theoretical limiting resolution of ~6 cm-1. The gratings stages are equipped with motors, one of which drives a lead screw, and the other is a motorized linear actuator, which gives both stages high angular resolution. The stage controlled by the lead screw has sufficient resolution to shift the spectrum of a particular analyte by a single pixel. The expected efficiency of this spectrometer is ~15%, which is comparable to the efficiency of other double spectrometers. The development of this spectrometer will allow the continuation of incisive studies of protein and peptide structure.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Wert, Jonathan D.jdw73@pitt.eduJDW73
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAsher, Sanford A.asher@pitt.eduASHER
Committee MemberSaxena, Sunilsksaxena@pitt.eduSKSAXENA
Committee MemberHorne, Sethhorne@pitt.eduHORNE
Committee MemberMadrua,
Date: 22 May 2014
Date Type: Publication
Defense Date: 17 April 2014
Approval Date: 22 May 2014
Submission Date: 18 April 2014
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 39
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Molecular Biophysics and Structural Biology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Raman, UV Resonance Raman, Spectrometer, Proteins, Signal-to-noise
Date Deposited: 22 May 2014 20:53
Last Modified: 15 Nov 2016 14:19


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