A Review of the Optical Control of Protein Function via Unnatural Amino Acid Mutagenesis and Other Optogenetic Approaches

Baker, Austin (2014) A Review of the Optical Control of Protein Function via Unnatural Amino Acid Mutagenesis and Other Optogenetic Approaches. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Biological processes are regulated with high spatial and temporal resolution at the
molecular, cellular, and systems level. To control and study processes with the same resolution,
researchers have employed light-sensitive moieties to optically activate and deactivate protein
function. Optical control is a non-invasive technique in which the amplitude, wavelength, spatial
location, and timing of the light illumination can be easily controlled. This review focuses on
applications of genetically encoded unnatural amino acids containing light-removable protecting
groups to optically trigger protein function, while also discussing select optogenetic approaches
using natural light-sensing domains to engineer optical control of biological processes.

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Details

Item Type:	University of Pittsburgh ETD
Status:	Unpublished
Creators/Authors:	Creators Email Pitt Username ORCID Baker, Austin asb93@pitt.edu ASB93
ETD Committee:	Title Member Email Address Pitt Username ORCID Committee Chair Deiters, Alex deiters@pitt.edu DEITERS Committee Member Horne, Seth horne@pitt.edu HORNE Committee Member Wipf, Peter pwipf@pitt.edu PWIPF
Date:	22 May 2014
Date Type:	Publication
Defense Date:	11 April 2014
Approval Date:	22 May 2014
Submission Date:	5 May 2014
Access Restriction:	No restriction; Release the ETD for access worldwide immediately.
Number of Pages:	48
Institution:	University of Pittsburgh
Schools and Programs:	Dietrich School of Arts and Sciences > Chemistry
Degree:	MS - Master of Science
Thesis Type:	Master's Thesis
Refereed:	Yes
Uncontrolled Keywords:	optogenetics, unnatural amino acids, light, protein control, UV light, expanded genetic code, mutagenesis
Date Deposited:	22 May 2014 18:18
Last Modified:	15 Nov 2016 14:20
URI:	http://d-scholarship.pitt.edu/id/eprint/21554

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