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Isolation of myogenic progenitor populations from Pax7-deficient skeletal muscle based on adhesion characteristics

Lu, A and Cummins, JH and Pollett, JB and Cao, B and Sun, B and Rudnicki, MA and Huard, J (2008) Isolation of myogenic progenitor populations from Pax7-deficient skeletal muscle based on adhesion characteristics. Gene Therapy, 15 (15). 1116 - 1125. ISSN 0969-7128

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Abstract

In an attempt to determine whether muscle-derived stem cells are distinct from satellite cells, we investigated whether muscle-derived stem cells could be isolated from the skeletal muscle of Pax7-deficient mice, which have been shown to be devoid of or to contain only a minimal number of satellite cells. Utilizing a technique that separates cells based on their adhesion characteristics (the preplate technique), several distinct populations of muscle-derived cells were isolated. In these mice, the Pax7 gene was knocked out with the insertion of the LacZ gene. One population was both rapidly adhering, LacZ-positive, and displayed a high myogenic index, but was rapidly lost to terminal differentiation when continuously replated. A second population, which persisted over 50 passages, was LacZ-negative and displayed a low myogenic index. Although Pax3 may have acted as a compensatory mechanism for the myogenic commitment of the LacZ-positive cells, the LacZ-negative cells, despite expressing Pax3, required Pax7 transduction to restore their myogenic capacity. We believe that these two populations of myogenic progenitor cells, each endowed with different adhesion characteristics, may help explain the discrepancy in the literature concerning the presence of myogenic cells found in Pax7-deficient mice.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lu, A
Cummins, JH
Pollett, JB
Cao, B
Sun, Bbis3@pitt.eduBIS3
Rudnicki, MA
Huard, J
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 August 2008
Date Type: Publication
Journal or Publication Title: Gene Therapy
Volume: 15
Number: 15
Page Range: 1116 - 1125
DOI or Unique Handle: 10.1038/gt.2008.86
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 0969-7128
MeSH Headings: Animals; Cell Culture Techniques; Cell Lineage; Cell Separation--methods; Cells, Cultured; Dystrophin--analysis; Flow Cytometry; Lac Operon; Mice; Mice, Knockout; Muscle, Skeletal--cytology; Muscle, Skeletal--metabolism; PAX7 Transcription Factor--genetics; PAX7 Transcription Factor--metabolism; Paired Box Transcription Factors--metabolism; Reverse Transcriptase Polymerase Chain Reaction--methods; Satellite Cells, Skeletal Muscle--metabolism; Stem Cells--cytology; Transduction, Genetic--methods
PubMed ID: 18509381
Date Deposited: 15 May 2014 20:35
Last Modified: 29 Jan 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/21591

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