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Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique

Nieponice, A and Soletti, L and Guan, J and Deasy, BM and Huard, J and Wagner, WR and Vorp, DA (2008) Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique. Biomaterials, 29 (7). 825 - 833. ISSN 0142-9612

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There is a clinical need for a tissue-engineered vascular graft (TEVG), and combining stem cells with biodegradable tubular scaffolds appears to be a promising approach. The goal of this study was to characterize the incorporation of muscle-derived stem cells (MDSCs) within tubular poly(ester urethane) urea (PEUU) scaffolds in vitro to understand their interaction, and to evaluate the mechanical properties of the constructs for vascular applications. Porous PEUU scaffolds were seeded with MDSCs using our recently described rotational vacuum seeding device, and cultured inside a spinner flask for 3 or 7 days. Cell viability, number, distribution and phenotype were assessed along with the suture retention strength and uniaxial mechanical behavior of the TEVGs. The seeding device allowed rapid even distribution of cells within the scaffolds. After 3 days, the constructs appeared completely populated with cells that were spread within the polymer. Cells underwent a population doubling of 2.1-fold, with a population doubling time of 35 h. Stem cell antigen-1 (Sca-1) expression by the cells remained high after 7 days in culture (77±20% vs. 66±6% at day 0) while CD34 expression was reduced (19±12% vs. 61±10% at day 0) and myosin heavy chain expression was scarce (not quantified). The estimated burst strength of the TEVG constructs was 2127±900 mmHg and suture retention strength was 1.3±0.3 N. We conclude from this study that MDSCs can be rapidly seeded within porous biodegradable tubular scaffolds while maintaining cell viability and high proliferation rates and without losing stem cell phenotype for up to 7 days of in-vitro culture. The successful integration of these steps is thought necessary to provide rapid availability of TEVGs, which is essential for clinical translation. © 2007 Elsevier Ltd. All rights reserved.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Nieponice, A
Soletti, L
Guan, J
Deasy, BM
Huard, J
Wagner, WRwagner@pitt.eduWAGNER
Vorp, DAvorp@pitt.eduVORP0000-0002-6467-2151
Date: 1 March 2008
Date Type: Publication
Journal or Publication Title: Biomaterials
Volume: 29
Number: 7
Page Range: 825 - 833
DOI or Unique Handle: 10.1016/j.biomaterials.2007.10.044
Schools and Programs: School of Medicine > Surgery
Refereed: Yes
ISSN: 0142-9612
MeSH Headings: Animals; Biocompatible Materials; Blood Vessel Prosthesis; Cell Separation; Cells, Cultured; Mice; Muscles--cytology; Stem Cells--cytology; Tissue Engineering--methods
Other ID: NLM NIHMS38590, NLM PMC2354918
PubMed Central ID: PMC2354918
PubMed ID: 18035412
Date Deposited: 15 May 2014 20:34
Last Modified: 02 Dec 2021 18:55


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