Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Microenvironments engineered by inkjet bioprinting spatially direct adult stem cells toward muscle- and bone-like subpopulations

Phillippi, JA and Miller, E and Weiss, L and Huard, J and Waggoner, A and Campbell, P (2008) Microenvironments engineered by inkjet bioprinting spatially direct adult stem cells toward muscle- and bone-like subpopulations. Stem Cells, 26 (1). 127 - 134. ISSN 1066-5099

[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

In vivo, growth factors exist both as soluble and as solid-phase molecules, immobilized to cell surfaces and within the extracellular matrix. We used this rationale to develop more biologically relevant approaches to study stem cell behaviors. We engineered stem cell microenvironments using inkjet bioprinting technology to create spatially defined patterns of immobilized growth factors. Using this approach, we engineered cell fate toward the osteogenic lineage in register to printed patterns of bone morphogenetic protein (BMP) 2 contained within a population of primary muscle-derived stem cells (MDSCs) isolated from adult mice. This patterning approach was conducive to patterning the MDSCs into subpopulations of osteogenic or myogenic cells simultaneously on the same chip. When cells were cultured under myogenic conditions on BMP-2 patterns, cells on pattern differentiated toward the osteogenic lineage, whereas cells off pattern differentiated toward the myogenic lineage. Time-lapse microscopy was used to visualize the formation of multinucleated myotubes, and immunocytochemistry was used to demonstrate expression of myosin heavy chain (fast) in cells off BMP-2 pattern. This work provides proof-of-concept for engineering spatially controlled multilineage differentiation of stem cells using patterns of immobilized growth factors. This approach may be useful for understanding cell behaviors to immobilized biological patterns and could have potential applications for regenerative medicine. ©AlphaMed Press.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Phillippi, JAjap103@pitt.eduJAP103
Miller, E
Weiss, L
Huard, J
Waggoner, A
Campbell, P
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 January 2008
Date Type: Publication
Journal or Publication Title: Stem Cells
Volume: 26
Number: 1
Page Range: 127 - 134
DOI or Unique Handle: 10.1634/stemcells.2007-0520
Schools and Programs: School of Medicine > Cardiothoracic Surgery
School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 1066-5099
Date Deposited: 15 May 2014 20:32
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/21594

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item