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Myogenic Endothelial Cells Purified From Human Skeletal Muscle Improve Cardiac Function After Transplantation Into Infarcted Myocardium

Okada, M and Payne, TR and Zheng, B and Oshima, H and Momoi, N and Tobita, K and Keller, BB and Phillippi, JA and Péault, B and Huard, J (2008) Myogenic Endothelial Cells Purified From Human Skeletal Muscle Improve Cardiac Function After Transplantation Into Infarcted Myocardium. Journal of the American College of Cardiology, 52 (23). 1869 - 1880. ISSN 0735-1097

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Objectives: The aim of this study was to evaluate the therapeutic potential of human skeletal muscle-derived myoendothelial cells for myocardial infarct repair. Background: We have recently identified and purified a novel population of myoendothelial cells from human skeletal muscle. These cells coexpress myogenic and endothelial cell markers and produce robust muscle regeneration when injected into cardiotoxin-injured skeletal muscle. Methods: Myoendothelial cells were isolated from biopsies of human skeletal muscle using a fluorescence-activated cell sorter along with populations of regular myoblasts and endothelial cells. Acute myocardial infarction was induced in male immune-deficient mice, and cells were directly injected into the ischemic area. Cardiac function was assessed by echocardiography, and donor cell engraftment, angiogenesis, scar tissue, endogenous cardiomyocyte proliferation, and apoptosis were all evaluated by immunohistochemistry. Results: A greater improvement in left ventricular function was observed after intramyocardial injection of myoendothelial cells when compared with that seen in hearts injected with myoblast or endothelial cells. Transplanted myoendothelial cells generated robust engraftments within the infarcted myocardium, and also stimulated angiogenesis, attenuation of scar tissue, and proliferation and survival of endogenous cardiomyocytes more effectively than transplanted myoblasts or endothelial cells. Conclusions: Our findings suggest that myoendothelial cells represent a novel cell population from human skeletal muscle that may hold promise for cardiac repair. © 2008 American College of Cardiology Foundation.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Okada, M
Payne, TR
Zheng, B
Oshima, H
Momoi, N
Tobita, Kkit3@pitt.eduKIT3
Keller, BB
Phillippi, JAjap103@pitt.eduJAP103
Péault, B
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 2 December 2008
Date Type: Publication
Journal or Publication Title: Journal of the American College of Cardiology
Volume: 52
Number: 23
Page Range: 1869 - 1880
DOI or Unique Handle: 10.1016/j.jacc.2008.07.064
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
School of Medicine > Pediatrics
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 0735-1097
MeSH Headings: Adult; Animals; Apoptosis; Cell Separation; Cell Transplantation--methods; Endothelial Cells--cytology; Female; Flow Cytometry; Humans; Male; Mice; Muscle, Skeletal--cytology; Muscle, Skeletal--metabolism; Muscle, Skeletal--pathology; Myoblasts--cytology; Myocardial Infarction--pathology; Myocardial Infarction--therapy; Myocardium--pathology; Vascular Endothelial Growth Factor A--metabolism
Other ID: NLM NIHMS80739, NLM PMC2719893
PubMed Central ID: PMC2719893
PubMed ID: 19038685
Date Deposited: 15 May 2014 20:29
Last Modified: 03 Feb 2019 01:55


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