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A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human Organs

Crisan, M and Yap, S and Casteilla, L and Chen, CW and Corselli, M and Park, TS and Andriolo, G and Sun, B and Zheng, B and Zhang, L and Norotte, C and Teng, PN and Traas, J and Schugar, R and Deasy, BM and Badylak, S and Buhring, HJ and Giacobino, JP and Lazzari, L and Huard, J and Péault, B (2008) A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human Organs. Cell Stem Cell, 3 (3). 301 - 313. ISSN 1934-5909

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Abstract

Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rβ expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells. © 2008 Elsevier Inc. All rights reserved.


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Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Crisan, M
Yap, S
Casteilla, L
Chen, CW
Corselli, M
Park, TS
Andriolo, G
Sun, Bbis3@pitt.eduBIS3
Zheng, B
Zhang, L
Norotte, C
Teng, PN
Traas, J
Schugar, R
Deasy, BM
Badylak, S
Buhring, HJ
Giacobino, JP
Lazzari, L
Huard, J
Péault, B
Centers: Other Centers, Institutes, or Units > McGowan Institute for Regenerative Medicine
Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 11 September 2008
Date Type: Publication
Journal or Publication Title: Cell Stem Cell
Volume: 3
Number: 3
Page Range: 301 - 313
DOI or Unique Handle: 10.1016/j.stem.2008.07.003
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 1934-5909
MeSH Headings: Adolescent; Adult; Adult Stem Cells--cytology; Aged; Cell Movement; Fetal Stem Cells--cytology; Fetus; Flow Cytometry; Humans; Mesenchymal Stromal Cells--cytology; Middle Aged; Muscle Development; Pericytes--cytology
PubMed ID: 18786417
Date Deposited: 15 May 2014 20:28
Last Modified: 04 Feb 2019 15:56
URI: http://d-scholarship.pitt.edu/id/eprint/21598

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