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The influence of sex on the chondrogenic potential of muscle-derived stem cells implications for cartilage regeneration and repair

Matsumoto, T and Kubo, S and Meszaros, LB and Corsi, KA and Cooper, GM and Li, G and Usas, A and Osawa, A and Fu, FH and Huard, J (2008) The influence of sex on the chondrogenic potential of muscle-derived stem cells implications for cartilage regeneration and repair. Arthritis and Rheumatism, 58 (12). 3809 - 3819. ISSN 0004-3591

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Objective. To explore possible differences in muscle-derived stem cell (MDSC) chondrogenic differentiation in vitro and articular cartilage regeneration in vivo between murine male MDSCs (M-MDSCs) and female MDSCs (F-MDSCs). Methods. Three different populations of M- and F-MDSCs (n = 3 of each sex) obtained via preplate technique, which separates cells based on their variable adhesion characteristics, were compared for their in vitro chondrogenic potential using pellet culture. Cells were assayed with and without retroviral transduction to express bone morphogenetic protein 4 (BMP-4). The influence of both expression of stem cell marker Sca1 and in vitro expansion on the chondrogenic potential of M- and F-MDSCs was also determined. Additionally, BMP-4-transduced M- and F-MDSCs were applied to a full-thickness articular cartilage defect (n = 5 each) on the femur of a nude rat, and the quality of the repaired tissue was evaluated by macroscopic and histologic examination. Results. With and without BMP-4 gene transduction, M-MDSCs produced significantly larger pellets with a richer extracellular matrix, compared with F-MDSCs. Sca1 purification influenced the chondrogenic potential of MDSCs, especially M-MDSCs. Long-term culture did not affect the chondrogenic potential of M-MDSCs but did influence F-MDSCs. M-MDSCs repaired articular cartilage defects more effectively than did F-MDSCs at all time points tested, as assessed both macroscopically and histologically. Conclusion. Our findings demonstrate that sex influences the chondrogenic differentiation and articular cartilage regeneration potential of MDSCs. Compared with female MDSCs, male MDSCs display more chondrogenic differentiation and better cartilage regeneration potential. © 2008, American College of Rheumatology.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Matsumoto, T
Kubo, S
Meszaros, LB
Corsi, KA
Cooper, GMgmc8@pitt.eduGMC8
Li, G
Usas, A
Osawa, A
Fu, FHffu@pitt.eduFFU
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 December 2008
Date Type: Publication
Journal or Publication Title: Arthritis and Rheumatism
Volume: 58
Number: 12
Page Range: 3809 - 3819
DOI or Unique Handle: 10.1002/art.24125
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Medicine > Biomedical Informatics
Refereed: Yes
ISSN: 0004-3591
MeSH Headings: Animals; Bone Morphogenetic Protein 4--genetics; Cartilage--cytology; Cartilage--physiology; Cell Differentiation--physiology; Cells, Cultured; Chondrogenesis--physiology; Female; Male; Mice; Mice, Inbred C57BL; Muscle, Skeletal--cytology; Nerve Tissue Proteins--metabolism; Nuclear Proteins--metabolism; Osteoarthritis--pathology; Osteoarthritis--physiopathology; Osteoarthritis--therapy; Rats; Rats, Nude; Regeneration--physiology; Sex Characteristics; Stem Cell Transplantation; Stem Cells--physiology; Tissue Engineering--methods; Transduction, Genetic
PubMed ID: 19035511
Date Deposited: 15 May 2014 20:25
Last Modified: 04 Feb 2019 15:56


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