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A role for cell sex in stem cell-mediated skeletal muscle regeneration: Female cells have higher muscle regeneration efficiency

Deasy, BM and Lu, A and Tebbets, JC and Feduska, JM and Schugar, RC and Pollett, JB and Sun, B and Urish, KL and Gharaibeh, BM and Cao, B and Rubin, RT and Huard, J (2007) A role for cell sex in stem cell-mediated skeletal muscle regeneration: Female cells have higher muscle regeneration efficiency. Journal of Cell Biology, 177 (1). 73 - 86. ISSN 0021-9525

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Abstract

We have shown that muscle-derived stem cells (MDSCs) transplanted into dystrophic (mdx) mice efficiently regenerate skeletal muscle. However, MDSC populations exhibit heterogeneity in marker profiles and variability in regeneration abilities. We show here that cell sex is a variable that considerably influences MDSCs' regeneration abilities. We found that the female MDSCs (F-MDSCs) regenerated skeletal muscle more efficiently. Despite using additional isolation techniques and cell cloning, we could not obtain a male subfraction with a regeneration capacity similar to that of their female counterparts. Rather than being directly hormonal or caused by host immune response, this difference in MDSCs' regeneration potential may arise from innate sex-related differences in the cells' stress responses. In comparison with F-MDSCs, male MDSCs have increased differentiation after exposure to oxidative stress induced by hydrogen peroxide, which may lead to in vivo donor cell depletion, and a proliferative advantage for F-MDSCs that eventually increases muscle regeneration. These findings should persuade researchers to report cell sex, which is a largely unexplored variable, and consider the implications of relying on cells of one sex. © The Rockefeller University Press.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Deasy, BM
Lu, A
Tebbets, JCJTEBBETS@pitt.eduJTEBBETS
Feduska, JM
Schugar, RC
Pollett, JB
Sun, Bbis3@pitt.eduBIS3
Urish, KLken.urish@pitt.eduKLU100000-0002-4511-1308
Gharaibeh, BMburhan@pitt.eduBURHAN0000-0002-5947-1232
Cao, B
Rubin, RT
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 9 April 2007
Date Type: Publication
Journal or Publication Title: Journal of Cell Biology
Volume: 177
Number: 1
Page Range: 73 - 86
DOI or Unique Handle: 10.1083/jcb.200612094
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 0021-9525
MeSH Headings: Animals; Cell Differentiation; Female; Gene Expression Profiling; Male; Mice; Mice, Inbred mdx; Muscle, Skeletal--cytology; Muscle, Skeletal--physiology; Oligonucleotide Array Sequence Analysis; Regeneration--genetics; Regeneration--physiology; Sex Factors; Stem Cell Transplantation; Stem Cells--classification; Stem Cells--physiology
Other ID: NLM PMC2064113
PubMed Central ID: PMC2064113
PubMed ID: 17420291
Date Deposited: 15 May 2014 20:15
Last Modified: 22 Jan 2021 17:55
URI: http://d-scholarship.pitt.edu/id/eprint/21607

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