Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Skeletal muscle fiber type conversion during the repair of mouse soleus: Potential implications for muscle healing after injury

Matsuura, T and Li, Y and Giacobino, JP and Fu, FH and Huard, J (2007) Skeletal muscle fiber type conversion during the repair of mouse soleus: Potential implications for muscle healing after injury. Journal of Orthopaedic Research, 25 (11). 1534 - 1540. ISSN 0736-0266

[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)


We used a mouse model of cardiotoxin injury to examine fiber type conversion during muscle repair. We evaluated the soleus muscles of 37 wild-type mice at 2, 4, 8, and 12 weeks after injury. We also used antibodies (fMHC and sMHC) against fast and slow myosin heavy chain to classify the myofibers into three categories: fast-, slow-, and mixed (hybrid)-type myofibers (myofibers expressing both fMHC and sMHC). Our results revealed an increase in the percentage of slow-type myofibers and a decrease in the percentage of fast-type myofibers during the repair process. The percentage of hybrid-type myofibers increased 2 weeks after injury, then gradually decreased over the following 6 weeks. Similarly, our analysis of centronucleated myofibers showed an increase in the percentage of slow-type myofibers and decreases in the percentages of fast-and hybrid-type myofibers. We also investigated the relationship between myofiber type conversion and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α). The expression of both PGC-1α protein, which is expressed in both the nucleus and the cytoplasm of regenerating myofibers, and sMHC protein increased with time after cardiotoxin injection, but we observed no significant differential expression of fMHC protein in regenerating muscle fibers during muscle repair. PGC-1α positive myofibers underwent fast to slow myofiber type conversion during the repair process. These results suggest that PGC-1α contributes to myofiber type conversion after muscle injury and that this phenomenon could influence the recovery of the injured muscle. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Matsuura, T
Li, Y
Giacobino, JP
Fu, FHffu@pitt.eduFFU
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 November 2007
Date Type: Publication
Journal or Publication Title: Journal of Orthopaedic Research
Volume: 25
Number: 11
Page Range: 1534 - 1540
DOI or Unique Handle: 10.1002/jor.20451
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 0736-0266
MeSH Headings: Animals; Biological Markers--metabolism; Blotting, Western; Cell Count; Female; Mice; Mice, Inbred C57BL; Muscle Fibers, Fast-Twitch--metabolism; Muscle Fibers, Fast-Twitch--pathology; Muscle Fibers, Slow-Twitch--metabolism; Muscle Fibers, Slow-Twitch--pathology; Muscle, Skeletal--injuries; Muscle, Skeletal--metabolism; Muscle, Skeletal--pathology; Regeneration; Trans-Activators--metabolism; Transcription Factors; Wound Healing
PubMed ID: 17593537
Date Deposited: 15 May 2014 20:12
Last Modified: 25 Jan 2019 23:55


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item