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Thermosensitive hydrogel PEG-PLGA-PEG enhances engraftment of muscle-derived stem cells and promotes healing in diabetic wound

Lee, PY and Cobain, E and Huard, J and Huang, L (2007) Thermosensitive hydrogel PEG-PLGA-PEG enhances engraftment of muscle-derived stem cells and promotes healing in diabetic wound. Molecular Therapy, 15 (6). 1189 - 1194. ISSN 1525-0016

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Abstract

Regenerating new tissue using cell transplantation has relied on successful cell engraftment in the host; however, cell engraftment into the diabetic skin wound is not as successful as in many other tissues. We used a biodegradable and biocompatible triblock co-polymer poly(ethylene glycol-b-[dl-lactic acid-co-glycolic acid]-b-ethylene glycol) (PEG-PLGA-PEG), which forms a thermosensitive hydrogel, as a wound dressing and scaffold. We found that the thermosensitive hydrogel increased the engraftment of muscle-derived stem cells (MDSCs) by 20- to 30-fold until day 20, when the wound was completely closed in a db/db genetically diabetic mouse model. At day 9, 30% of the transplanted MDSCs were found to remain, and 15% remained at day 20 after transplantation. The increased engraftment resulted in enhanced wound healing, as indicated by the wound closure rate, epithelium migration, and collagen deposition. Using MDSCs stably expressing β-gal and immunofluorescence, we found that 25% of MDSCs differentiated into fibroblasts, 10% into myofibroblasts, and 10% into endothelial cells. We conclude that using the thermosensitive hydrogel as a scaffold increased the engraftment of MDSCs, which leads to improved diabetic wound healing, possibly by retaining the cells at the wound site for longer.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lee, PY
Cobain, E
Huard, J
Huang, L
Date: 1 June 2007
Date Type: Publication
Journal or Publication Title: Molecular Therapy
Volume: 15
Number: 6
Page Range: 1189 - 1194
DOI or Unique Handle: 10.1038/sj.mt.6300156
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Pharmacy > Pharmaceutical Sciences
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 1525-0016
MeSH Headings: Animals; Cell Differentiation; Cells, Cultured; Collagen--metabolism; Dermatologic Surgical Procedures; Diabetes Mellitus--physiopathology; Diabetes Mellitus--surgery; Female; Fluorescent Antibody Technique; Hydrogels; Lactic Acid--chemistry; Mice; Mice, Inbred C57BL; Myoblasts--cytology; Myoblasts--transplantation; Polyethylene Glycols--chemistry; Polyglycolic Acid--chemistry; Polymers--chemistry; Skin--metabolism; Skin--pathology; Stem Cell Transplantation--methods; Wound Healing
PubMed ID: 17406344
Date Deposited: 09 Jun 2014 14:45
Last Modified: 29 Jan 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/21667

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