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Prospective identification of myogenic endothelial cells in human skeletal muscle

Zheng, B and Cao, B and Crisan, M and Sun, B and Li, G and Logar, A and Yap, S and Pollett, JB and Drowley, L and Cassino, T and Gharaibeh, B and Deasy, BM and Huard, J and Péault, B (2007) Prospective identification of myogenic endothelial cells in human skeletal muscle. Nature Biotechnology, 25 (9). 1025 - 1034. ISSN 1087-0156

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We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56+ myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56+ myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease. © 2007 Nature Publishing Group.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Zheng, B
Cao, B
Crisan, M
Sun, Bbis3@pitt.eduBIS3
Li, G
Logar, A
Yap, S
Pollett, JB
Drowley, L
Cassino, T
Gharaibeh, Bburhan@pitt.eduBURHAN0000-0002-5947-1232
Deasy, BM
Huard, J
Péault, B
Centers: Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine
Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 September 2007
Date Type: Publication
Journal or Publication Title: Nature Biotechnology
Volume: 25
Number: 9
Page Range: 1025 - 1034
DOI or Unique Handle: 10.1038/nbt1334
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
School of Medicine > Pediatrics
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 1087-0156
MeSH Headings: Adolescent; Adult; Aged; Animals; Antigens, CD56; Biological Markers--metabolism; Cell Proliferation; Cell Survival; Cells, Cultured; Child; Clone Cells; Endothelial Cells--cytology; Flow Cytometry; Humans; Mice; Mice, SCID; Middle Aged; Muscle, Skeletal--cytology; Muscle, Skeletal--physiology; Neoplasms--pathology; Regeneration; Time Factors
PubMed ID: 17767154
Date Deposited: 09 Jun 2014 14:44
Last Modified: 22 Jun 2021 11:55


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