Zheng, B and Cao, B and Crisan, M and Sun, B and Li, G and Logar, A and Yap, S and Pollett, JB and Drowley, L and Cassino, T and Gharaibeh, B and Deasy, BM and Huard, J and Péault, B
(2007)
Prospective identification of myogenic endothelial cells in human skeletal muscle.
Nature Biotechnology, 25 (9).
1025 - 1034.
ISSN 1087-0156
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Abstract
We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56+ myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56+ myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease. © 2007 Nature Publishing Group.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Zheng, B | | | | Cao, B | | | | Crisan, M | | | | Sun, B | bis3@pitt.edu | BIS3 | | Li, G | | | | Logar, A | | | | Yap, S | | | | Pollett, JB | | | | Drowley, L | | | | Cassino, T | | | | Gharaibeh, B | burhan@pitt.edu | BURHAN | 0000-0002-5947-1232 | Deasy, BM | | | | Huard, J | | | | Péault, B | | | |
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Centers: |
Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine Other Centers, Institutes, Offices, or Units > Stem Cell Research Center |
Date: |
1 September 2007 |
Date Type: |
Publication |
Journal or Publication Title: |
Nature Biotechnology |
Volume: |
25 |
Number: |
9 |
Page Range: |
1025 - 1034 |
DOI or Unique Handle: |
10.1038/nbt1334 |
Schools and Programs: |
School of Medicine > Biochemistry and Molecular Genetics School of Medicine > Orthopaedic Surgery School of Medicine > Pediatrics Swanson School of Engineering > Bioengineering |
Refereed: |
Yes |
ISSN: |
1087-0156 |
MeSH Headings: |
Adolescent; Adult; Aged; Animals; Antigens, CD56; Biological Markers--metabolism; Cell Proliferation; Cell Survival; Cells, Cultured; Child; Clone Cells; Endothelial Cells--cytology; Flow Cytometry; Humans; Mice; Mice, SCID; Middle Aged; Muscle, Skeletal--cytology; Muscle, Skeletal--physiology; Neoplasms--pathology; Regeneration; Time Factors |
PubMed ID: |
17767154 |
Date Deposited: |
09 Jun 2014 14:44 |
Last Modified: |
22 Jun 2021 11:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/21669 |
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