Mouse adipose-derived stem cells undergo multilineage differentiation in vitro but primarily osteogenic and chondrogenic differentiation in vivo

Zheng, B and Cao, B and Li, G and Huard, J (2006) Mouse adipose-derived stem cells undergo multilineage differentiation in vitro but primarily osteogenic and chondrogenic differentiation in vivo. Tissue Engineering, 12 (7). 1891 - 1901. ISSN 1076-3279

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Abstract

Human, rat, and mouse studies have demonstrated the existence of a population of adipose-derived adult stem (ADAS) cells that can undergo multilineage differentiation in vitro. However, it remains unclear whether these cells maintain their multilineage potential in vivo. The aim of this study was to examine the in vitro and in vivo characteristics and behavior of a potential population of murine ADAS (muADAS) cells isolated from the visceral fat of the abdominal cavity of C57BL/10J mice. We used flow cytometry to examine the cells' expression of CD29, CD31, CD45, CD34, CD44, CD144, CD146, Flk1, and Sca-1. The isolated cell population was CD45 negative, which precludes contamination by hematopoietic cells, but was partially positive for Sca-1 and CD34: 2 stem-cell markers. After induction in conditioned medium, the muADAS cells gained the ability to undergo adipogenic, osteogenic, chondrogenic, myogenic, and hematopoietic differentiation in vitro. The muADAS cells readily differentiated to form bone and cartilage in vivo for up to 24 weeks, but their ability to regenerate muscle or reconstitute bone marrow was found to be limited. © Mary Ann Liebert, Inc.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Zheng, B Cao, B Li, G Huard, J
Date:	1 July 2006
Date Type:	Publication
Journal or Publication Title:	Tissue Engineering
Volume:	12
Number:	7
Page Range:	1891 - 1901
DOI or Unique Handle:	10.1089/ten.2006.12.1891
Schools and Programs:	School of Medicine > Orthopaedic Surgery
Refereed:	Yes
ISSN:	1076-3279
Related URLs:	Publisher Web site
MeSH Headings:	Adipose Tissue--cytology; Adipose Tissue--physiology; Animals; Antigens, CD--metabolism; Cell Differentiation--physiology; Cells, Cultured; Chondrogenesis--physiology; Mice; Mice, SCID; Osteogenesis--physiology; Stem Cells--cytology; Stem Cells--physiology; Time Factors
PubMed ID:	16889519
Date Deposited:	09 Jun 2014 14:50
Last Modified:	29 Jan 2019 15:55
URI:	http://d-scholarship.pitt.edu/id/eprint/21679

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