Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Periurethral cellular injection: Comparison of muscle-derived progenitor cells and fibroblasts with regard to efficacy and tissue contractility in an animal model of stress urinary incontinence

Kwon, D and Kim, Y and Pruchnic, R and Jankowski, R and Usiene, I and de Miguel, F and Huard, J and Chancellor, MB (2006) Periurethral cellular injection: Comparison of muscle-derived progenitor cells and fibroblasts with regard to efficacy and tissue contractility in an animal model of stress urinary incontinence. Urology, 68 (2). 449 - 454. ISSN 0090-4295

[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

Objectives: To compare muscle-derived cells (MDCs) and fibroblasts with regard to their potential for restoration of urethral function on injection in a previously established animal model of stress urinary incontinence. Methods: The animals were divided into four (dosage) or five (cell concentration) experimental groups: normal, nontreated controls (normal group) or bilateral sciatic nerve transection with either periurethral injection of saline (saline group), MDCs (MDC group), fibroblasts (fibroblast group), or MDC/fibroblast mixture (mixed group). At 4 weeks after injection, the leak point pressure (LPP) was measured and contractility testing and histologic analysis were performed. Results: The histologic examination demonstrated muscular atrophy in the saline group and new striated muscle fibers at the sites of MDC injection in the MDC group, but not in the fibroblast group. Denervation of the urethra resulted in a significant decrease of maximal fast-twitch muscle contraction amplitude to only 9% of normal. MDC injection into the denervated urethra significantly improved the fast-twitch muscle contraction amplitude to 73% of normal. The LPP of the normal, saline, MDC, fibroblast, and mixed groups at 4 weeks after treatment was 43.3 ± 2.5, 25.8 ± 1.4, 38.2 ± 4.2, 38.3 ± 1.2, and 34.5 ± 3.3 cm H2O, respectively. In the cell dosage experiment, the LPP increased with increases in the injected cell number. Evidence of obstruction was observed in the high-dose (1 × 107 cells) fibroblast group. Conclusions: Although both MDCs and fibroblast injection increased the LPP in a stress urinary incontinence rat model, only MDCs significantly improved urethral muscle strip contractility. Moreover, urinary retention developed with high-dose fibroblast injection, but not with MDC injection. © 2006 Elsevier Inc. All rights reserved.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Kwon, D
Kim, Y
Pruchnic, R
Jankowski, R
Usiene, I
de Miguel, F
Huard, J
Chancellor, MB
Date: 1 August 2006
Date Type: Publication
Journal or Publication Title: Urology
Volume: 68
Number: 2
Page Range: 449 - 454
DOI or Unique Handle: 10.1016/j.urology.2006.03.040
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Medicine > Urology
Refereed: Yes
ISSN: 0090-4295
MeSH Headings: Animals; Disease Models, Animal; Female; Fibroblasts; Injections; Muscle Contraction; Rats; Rats, Sprague-Dawley; Stem Cells; Urinary Incontinence, Stress--physiopathology; Urinary Incontinence, Stress--therapy
PubMed ID: 16904482
Date Deposited: 09 Jun 2014 14:49
Last Modified: 29 Nov 2019 22:55
URI: http://d-scholarship.pitt.edu/id/eprint/21680

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item