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VEGF and BMP expression in mouse osteosarcoma cells

Weiss, KR and Cooper, GM and Jadlowiec, JA and McGough, RL and Huard, J (2006) VEGF and BMP expression in mouse osteosarcoma cells. Clinical Orthopaedics and Related Research (450). 111 - 117. ISSN 0009-921X

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Abstract

Osteosarcoma is the most common primary bone malignancy. Despite improvements in therapy, approximately 30% of patients experience pulmonary metastasis. Expression of several growth factors, including VEGF and BMPs, has been implicated in tumor progression and metastatic potential. We hypothesized increased metastatic potential of mouse osteosarcoma cells positively correlates with the expression of VEGF and BMPs. We studied the expression patterns of these growth factors in two murine osteosarcoma cell lines with varying degrees of metastatic potential: K7M2 (highly metastatic) and K12 (minimally metastatic). Expression of VEGF and BMP2 were higher in the metastatic K7M2 cell line. We also investigated the effects of the BMP antagonist noggin on osteosarcoma growth characteristics in vitro. We noted decreased motility, altered morphology, and increased cell death in the highly metastatic K7M2 cell line. Less metastatic K12 cells showed substantial cell death without clear alteration of motility or morphology. These data suggest BMP2 expression may be an important factor in osteosarcoma metastasis and noggin administration theoretically could block its actions. Inhibition of BMPs and VEGF should be investigated further as a possible strategy for decreasing the incidence of pulmonary metastases in osteosarcoma. © 2006 Lippincott Williams & Wilkins.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Weiss, KRkrw13@pitt.eduKRW13
Cooper, GMgmc8@pitt.eduGMC8
Jadlowiec, JA
McGough, RLrlm28@pitt.eduRLM28
Huard, J
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 1 January 2006
Date Type: Publication
Journal or Publication Title: Clinical Orthopaedics and Related Research
Number: 450
Page Range: 111 - 117
DOI or Unique Handle: 10.1097/01.blo.0000229333.98781.56
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 0009-921X
MeSH Headings: Animals; Bone Morphogenetic Proteins--metabolism; Cell Line, Tumor; Mice; Mice, Inbred BALB C; Osteosarcoma--metabolism; Vascular Endothelial Growth Factor A--metabolism
PubMed ID: 16906080
Date Deposited: 09 Jun 2014 14:48
Last Modified: 12 Mar 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/21683

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