Shen, W and Prisk, V and Li, Y and Foster, W and Huard, J
(2006)
Inhibited skeletal muscle healing in cyclooxygenase-2 gene-deficient mice: The role of PGE<inf>2</inf> and PGF<inf>2α</inf>.
Journal of Applied Physiology, 101 (4).
1215 - 1221.
ISSN 8750-7587
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Abstract
Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat skeletal muscle injury. However, studies have shown that NSAIDs may be detrimental to the healing process. Mediated by prostaglandin F 2α (PGF2α) and prostaglandin E2 (PGE2), the cycloxygenase-2 (COX-2) pathway plays an important role in muscle healing. We hypothesize that the COX-2 pathway is important for the fusion of muscle cells and the regeneration of injured muscle. For the in vitro experiments, we isolated myogenic precursor cells from wild-type (Wt) and COX-2 gene-deficient (COX-2-/-) mice and examined the effect of PGE 2 and PGF2α on cell fusion. For the in vivo experiments, we created laceration injury on the tibialis anterior (TA) muscles of Wt and COX-2-/- mice. Five and 14 days after injury, we examined the TA muscles histologically and functionally. We found that the secondary fusion between nascent myotubes and myogenic precursor cells isolated from COX-2-/- mice was severely compromised compared with that of Wt controls but was restored by the addition of PGF2α or, to a lesser extent, PGE2 to the culture. Histological and functional assessments of the TA muscles in COX-2-/- mice revealed decreased regeneration relative to that observed in the Wt mice. The findings reported here demonstrate that the COX-2 pathway plays an important role in muscle healing and that prostaglandins are key mediators of the COX-2 pathway. It suggests that the decision to use NSAIDs to treat muscle injuries warrants critical evaluation because NSAIDs might impair muscle healing by inhibiting the fusion of myogenic precursor cells. Copyright © 2006 the American Physiological Society.
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Details
| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Shen, W | | | | | Prisk, V | | | | | Li, Y | | | | | Foster, W | | | | | Huard, J | | | |
|
| Date: |
9 October 2006 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Journal of Applied Physiology |
| Volume: |
101 |
| Number: |
4 |
| Page Range: |
1215 - 1221 |
| DOI or Unique Handle: |
10.1152/japplphysiol.01331.2005 |
| Schools and Programs: |
School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
Swanson School of Engineering > Bioengineering |
| Refereed: |
Yes |
| ISSN: |
8750-7587 |
| MeSH Headings: |
Animals; Cell Fusion; Cell Movement--drug effects; Cell Survival--drug effects; Cells, Cultured; Cyclooxygenase 2--deficiency; Cyclooxygenase 2--genetics; Dinoprost--pharmacology; Dinoprostone--pharmacology; Dose-Response Relationship, Drug; Mice; Mice, Knockout; Muscle Fibers, Skeletal--drug effects; Muscle Fibers, Skeletal--metabolism; Muscle Fibers, Skeletal--pathology; Muscle, Skeletal--drug effects; Muscle, Skeletal--metabolism; Muscle, Skeletal--pathology; Myoblasts--drug effects; Myoblasts--metabolism; Myoblasts--pathology; Wound Healing--drug effects; Wound Healing--physiology |
| PubMed ID: |
16778000 |
| Date Deposited: |
09 Jun 2014 14:46 |
| Last Modified: |
11 Jun 2021 22:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/21693 |
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