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A novel outbred mouse model of 2009 pandemic influenza and bacterial co-infection severity

McHugh, KJ and Mandalapu, S and Kolls, JK and Ross, TM and Alcorn, JF (2013) A novel outbred mouse model of 2009 pandemic influenza and bacterial co-infection severity. PLoS ONE, 8 (12).

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Abstract

Influenza viruses pose a significant health risk and annually impose a great cost to patients and the health care system. The molecular determinants of influenza severity, often exacerbated by secondary bacterial infection, are largely unclear. We generated a novel outbred mouse model of influenza virus, Staphylococcus aureus, and coinfection utilizing influenza A/CA/07/2009 virus and S. aureus (USA300). Outbred mice displayed a wide range of pathologic phenotypes following influenza virus or co-infection ranging broadly in severity. Influenza viral burden positively correlated with weight loss although lung histopathology did not. Inflammatory cytokines including IL-6, TNF-α, G-CSF, and CXCL10 positively correlated with both weight loss and viral burden. In S. aureus infection, IL-1β, G-CSF, TNF-α, and IL-6 positively correlated with weight loss and bacterial burden. In co-infection, IL-1β production correlated with decreased weight loss suggesting a protective role. The data demonstrate an approach to identify biomarkers of severe disease and to understand pathogenic mechanisms in pneumonia. © 2013 McHugh et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
McHugh, KJkjmst65@pitt.eduKJMST65
Mandalapu, S
Kolls, JKjkk23@pitt.eduJKK23
Ross, TM
Alcorn, JFjfa9@pitt.eduJFA9
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorCormier, Stephania AnnUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, or Units > Center for Vaccine Research
Date: 6 December 2013
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 8
Number: 12
DOI or Unique Handle: 10.1371/journal.pone.0082865
Schools and Programs: School of Medicine > Microbiology and Molecular Genetics
Refereed: Yes
Date Deposited: 13 Jun 2014 19:12
Last Modified: 02 Feb 2019 16:57
URI: http://d-scholarship.pitt.edu/id/eprint/21845

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