Fazzari, M and Trostchansky, A and Schopfer, FJ and Salvatore, SR and Sánchez-Calvo, B and Vitturi, D and Valderrama, R and Barroso, JB and Radi, R and Freeman, BA and Rubbo, H
(2014)
Olives and olive oil are sources of electrophilic fatty acid nitroalkenes.
PLoS ONE, 9 (1).
Abstract
Extra virgin olive oil (EVOO) and olives, key sources of unsaturated fatty acids in the Mediterranean diet, provide health benefits to humans. Nitric oxide (•NO) and nitrite (NO2-)-dependent reactions of unsaturated fatty acids yield electrophilic nitroalkene derivatives (NO 2-FA) that manifest salutary pleiotropic cell signaling responses in mammals. Herein, the endogenous presence of NO2-FA in both EVOO and fresh olives was demonstrated by mass spectrometry. The electrophilic nature of these species was affirmed by the detection of significant levels of protein cysteine adducts of nitro-oleic acid (NO2-OA-cysteine) in fresh olives, especially in the peel. Further nitration of EVOO by NO2- under acidic gastric digestive conditions revealed that human consumption of olive lipids will produce additional nitro-conjugated linoleic acid (NO2-cLA) and nitro-oleic acid (NO2-OA). The presence of free and protein-adducted NO2-FA in both mammalian and plant lipids further affirm a role for these species as signaling mediators. Since NO2-FA instigate adaptive anti-inflammatory gene expression and metabolic responses, these redox-derived metabolites may contribute to the cardiovascular benefits associated with the Mediterranean diet. © 2014 Fazzari et al.
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Fazzari, M | maf167@pitt.edu | MAF167 | | | Trostchansky, A | | | | | Schopfer, FJ | fjschopfer@katz.pitt.edu | FJS2 | | | Salvatore, SR | srs107@pitt.edu | SRS107 | | | Sánchez-Calvo, B | | | | | Vitturi, D | dav28@pitt.edu | DAV28 | 0000-0003-0354-4567 | | Valderrama, R | | | | | Barroso, JB | | | | | Radi, R | | | | | Freeman, BA | freerad@pitt.edu | FREERAD | | | Rubbo, H | | | |
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| Contributors: |
| Contribution | Contributors Name | Email | Pitt Username | ORCID |
|---|
| Editor | Sladek, Frances M. | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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| Date: |
14 January 2014 |
| Date Type: |
Publication |
| Journal or Publication Title: |
PLoS ONE |
| Volume: |
9 |
| Number: |
1 |
| DOI or Unique Handle: |
10.1371/journal.pone.0084884 |
| Schools and Programs: |
School of Medicine > Pharmacology and Chemical Biology |
| Refereed: |
Yes |
| Date Deposited: |
16 Jun 2014 17:30 |
| Last Modified: |
22 Jun 2021 12:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/21870 |
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