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Impact of chemotherapy for HIV-1 related lymphoma on residual viremia and cellular HIV-1 DNA in patients on suppressive antiretroviral therapy

Cillo, AR and Krishnan, S and McMahon, DK and Mitsuyasu, RT and Para, MF and Mellors, JW (2014) Impact of chemotherapy for HIV-1 related lymphoma on residual viremia and cellular HIV-1 DNA in patients on suppressive antiretroviral therapy. PLoS ONE, 9 (3).

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Abstract

The first cure of HIV-1 infection was achieved through complex, multimodal therapy including myeloablative chemotherapy, total body irradiation, anti-Thymocyte globulin, and allogeneic stem cell transplantation with a CCR5 delta32 homozygous donor. The contributions of each component of this therapy to HIV-1 eradication are unclear. To assess the impact of cytotoxic chemotherapy alone on HIV-1 persistence, we longitudinally evaluated low-level plasma viremia and HIV-1 DNA in PBMC from patients in the ACTG A5001/ALLRT cohort on suppressive antiretroviral therapy (ART) who underwent chemotherapy for HIV-1 related lymphoma without interrupting ART. Plasma HIV-1 RNA, total HIV-1 DNA and 2-LTR circles (2-LTRs) in PBMC were measured using sensitive qPCR assays. In the 9 patients who received moderately intensive chemotherapy for HIV-1 related lymphoma with uninterrupted ART, low-level plasma HIV-1 RNA did not change significantly with chemotherapy: median HIV-1 RNA was 1 copy/mL (interquartile range: 1.0 to 20) pre-chemotherapy versus 4 copies/mL (interquartile range: 1.0 to 7.0) post-chemotherapy. HIV-1 DNA levels also did not change significantly, with median prechemotherapy HIV-1 DNA of 355 copies/106 CD4+ cells versus 228 copies/106 CD4+ cells post-chemotherapy. 2-LTRs were detectable in 2 of 9 patients pre-chemotherapy and in 3 of 9 patients post-chemotherapy. In summary, moderately intensive chemotherapy for HIV-1 related lymphoma in the context of continuous ART did not have a prolonged impact on HIV-1 persistence. © 2014 Cillo et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Cillo, ARarc85@pitt.eduARC85
Krishnan, S
McMahon, DK
Mitsuyasu, RT
Para, MF
Mellors, JWjwm1@pitt.eduJWM1
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorGraham, Susan MarieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 17 March 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0092118
Schools and Programs: School of Medicine > Medicine
Refereed: Yes
Date Deposited: 30 Jun 2014 16:01
Last Modified: 01 Mar 2019 15:01
URI: http://d-scholarship.pitt.edu/id/eprint/21943

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