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A Non-Invasive method of quantifying pancreatic volume in mice using micro-MRI

Paredes, JL and Orabi, AI and Ahmad, T and Benbourenane, I and Tobita, K and Tadros, S and Bae, KT and Husain, SZ (2014) A Non-Invasive method of quantifying pancreatic volume in mice using micro-MRI. PLoS ONE, 9 (3).

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In experimental models of pancreatic growth and recovery, changes in pancreatic size are assessed by euthanizing a large cohort of animals at varying time points and measuring organ mass. However, to ascertain this information in clinical practice, patients with pancreatic disorders routinely undergo non-invasive cross-sectional imaging of the pancreas using magnetic resonance imaging (MRI) or computed tomography (CT). The aim of the current study was to develop a thinsliced, optimized sequence protocol using a high field MRI to accurately calculate pancreatic volumes in the most common experimental animal, the mouse. Using a 7 Telsa Bruker micro-MRI system, we performed abdominal imaging in whole-fixed mice in three standard planes: axial, sagittal, and coronal. The contour of the pancreas was traced using Vitrea software and then transformed into a 3-dimensional (3D) reconstruction, from which volumetric measurements were calculated. Images were optimized using heart perfusion-fixation, T1 sequence analysis, and 0.2 to 0.4 mm thick slices. As proof of principle, increases in pancreatic volume among mice of different ages correlated tightly with increasing body weight. In summary, this is the first study to measure pancreatic volumes in mice, using a high field 7 Tesla micro-MRI and a thin-sliced, optimized sequence protocol. We anticipate that micro-MRI will improve the ability to non-invasively quantify changes in pancreatic size and will dramatically reduce the number of animals required to serially assess pancreatic growth and recovery.© 2014 Paredes et al.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Paredes, JL
Orabi, AI
Ahmad, T
Benbourenane, Iilb8@pitt.eduILB8
Tobita, Kkit3@pitt.eduKIT3
Tadros, Ssst13@pitt.eduSST13
Bae, KTktb4@pitt.eduKTB4
Husain, SZsoh14@pitt.eduSOH140000-0001-9916-319X
ContributionContributors NameEmailPitt UsernameORCID
Date: 18 March 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0092263
Schools and Programs: School of Medicine > Developmental Biology
School of Medicine > Pediatrics
School of Medicine > Radiology
Refereed: Yes
Date Deposited: 30 Jun 2014 16:02
Last Modified: 23 Jan 2018 21:55


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