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Clearance kinetics and matrix binding partners of the receptor for advanced glycation end products

Milutinovic, PS and Englert, JM and Crum, LT and Mason, NS and Ramsgaard, L and Enghild, JJ and Sparvero, LJ and Lotze, MT and Oury, TD (2014) Clearance kinetics and matrix binding partners of the receptor for advanced glycation end products. PLoS ONE, 9 (3).

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Abstract

Elucidating the sites and mechanisms of sRAGE action in the healthy state is vital to better understand the biological importance of the receptor for advanced glycation end products (RAGE). Previous studies in animal models of disease have demonstrated that exogenous sRAGE has an anti-inflammatory effect, which has been reasoned to arise from sequestration of pro-inflammatory ligands away from membrane-bound RAGE isoforms. We show here that sRAGE exhibits in vitro binding with high affinity and reversibly to extracellular matrix components collagen I, collagen IV, and laminin. Soluble RAGE administered intratracheally, intravenously, or intraperitoneally, does not distribute in a specific fashion to any healthy mouse tissue, suggesting against the existence of accessible sRAGE sinks and receptors in the healthy mouse. Intratracheal administration is the only effective means of delivering exogenous sRAGE to the lung, the organ in which RAGE is most highly expressed; clearance of sRAGE from lung does not differ appreciably from that of albumin. Copyright: © 2014 Milutinovic et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Milutinovic, PS
Englert, JM
Crum, LT
Mason, NSmasons@pitt.eduMASONS
Ramsgaard, L
Enghild, JJ
Sparvero, LJsparverolj@pitt.eduLJS39
Lotze, MTmtl5@pitt.eduMTL5
Oury, TDtdoury@pitt.eduTDOURY
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
DirectorAbrams, William R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 18 March 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0088259
Schools and Programs: School of Medicine > Pathology
School of Medicine > Radiology
School of Medicine > Surgery
Refereed: Yes
Date Deposited: 30 Jun 2014 15:59
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/21947

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