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Analysis of vaginal microbicide film hydration kinetics by quantitative imaging refractometry

Rinehart, M and Grab, S and Rohan, L and Katz, D and Wax, A (2014) Analysis of vaginal microbicide film hydration kinetics by quantitative imaging refractometry. PLoS ONE, 9 (4).

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Abstract

We have developed a quantitative imaging refractometry technique, based on holographic phase microscopy, as a tool for investigating microscopic structural changes in water-soluble polymeric materials. Here we apply the approach to analyze the structural degradation of vaginal topical microbicide films due to water uptake. We implemented transmission imaging of 1-mm diameter film samples loaded into a flow chamber with a 1.5×2 mm field of view. After water was flooded into the chamber, interference images were captured and analyzed to obtain high resolution maps of the local refractive index and subsequently the volume fraction and mass density of film material at each spatial location. Here, we compare the hydration dynamics of a panel of films with varying thicknesses and polymer compositions, demonstrating that quantitative imaging refractometry can be an effective tool for evaluating and characterizing the performance of candidate microbicide film designs for anti-HIV drug delivery. © 2014 Rinehart et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Rinehart, M
Grab, Ssmgrab@pitt.eduSMGRAB
Rohan, Lrohanl@pitt.eduROHANL
Katz, D
Wax, A
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorXu, BingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 15 April 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 4
DOI or Unique Handle: 10.1371/journal.pone.0095005
Schools and Programs: School of Pharmacy > Pharmaceutical Sciences
Refereed: Yes
Date Deposited: 23 Jun 2014 20:56
Last Modified: 02 Feb 2019 14:56
URI: http://d-scholarship.pitt.edu/id/eprint/21980

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