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Reproducibility of in-vivo OCT measured three-dimensional human lamina cribrosa microarchitecture

Wang, B and Nevins, JE and Nadler, Z and Wollstein, G and Ishikawa, H and Bilonick, RA and Kagemann, L and Sigal, IA and Grulkowski, I and Liu, JJ and Kraus, M and Lu, CD and Hornegger, J and Fujimoto, JG and Schuman, JS (2014) Reproducibility of in-vivo OCT measured three-dimensional human lamina cribrosa microarchitecture. PLoS ONE, 9 (4).

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Purpose: To determine the reproducibility of automated segmentation of the three-dimensional (3D) lamina cribrosa (LC) microarchitecture scanned in-vivo using optical coherence tomography (OCT). Methods: Thirty-nine eyes (8 healthy, 19 glaucoma suspects and 12 glaucoma) from 49 subjects were scanned twice using swept-source (SS-) OCT in a 3.5x3.5x3.64 mm (400x400x896 pixels) volume centered on the optic nerve head, with the focus readjusted after each scan. The LC was automatically segmented and analyzed for microarchitectural parameters, including pore diameter, pore diameter standard deviation (SD), pore aspect ratio, pore area, beam thickness, beam thickness SD, and beam thickness to pore diameter ratio. Reproducibility of the parameters was assessed by computing the imprecision of the parameters between the scans. Results: The automated segmentation demonstrated excellent reproducibility. All LC microarchitecture parameters had an imprecision of less or equal to 4.2%. There was little variability in imprecision with respect to diagnostic category, although the method tends to show higher imprecision amongst healthy subjects. Conclusion: The proposed automated segmentation of the LC demonstrated high reproducibility for 3D LC parameters. This segmentation analysis tool will be useful for in-vivo studies of the LC. © 2014 Wang et al.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Wang, Bbow8@pitt.eduBOW8
Nevins, JE
Nadler, Z
Wollstein, Gchw28@pitt.eduCHW28
Ishikawa, Hhii3@pitt.eduHII3
Bilonick, RArab45@pitt.eduRAB45
Kagemann, Llek19@pitt.eduLEK19
Sigal, IAian.sigal@pitt.eduIAS6
Grulkowski, I
Liu, JJ
Kraus, M
Lu, CD
Hornegger, J
Fujimoto, JG
Schuman, JSjss28@pitt.eduJSS280000-0002-8885-3766
ContributionContributors NameEmailPitt UsernameORCID
Date: 18 April 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 4
DOI or Unique Handle: 10.1371/journal.pone.0095526
Schools and Programs: School of Public Health > Biostatistics
School of Medicine > Ophthalmology
Swanson School of Engineering > Bioengineering
Refereed: Yes
Date Deposited: 23 Jun 2014 21:03
Last Modified: 02 Feb 2019 16:56


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