S100A8 Contributes to drug resistance by promoting autophagy in leukemia cells

Yang, M and Zeng, P and Kang, R and Yu, Y and Yang, L and Tang, D and Cao, L (2014) S100A8 Contributes to drug resistance by promoting autophagy in leukemia cells. PLoS ONE, 9 (5).

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Abstract

Autophagy is a double-edged sword in tumorigenesis and plays an important role in the resistance of cancer cells to chemotherapy. S100A8 is a member of the S100 calcium-binding protein family and plays an important role in the drug resistance of leukemia cells, with the mechanisms largely unknown. Here we report that S100A8 contributes to drug resistance in leukemia by promoting autophagy. S100A8 level was elevated in drug resistance leukemia cell lines relative to the nondrug resistant cell lines. Adriamycin and vincristine increased S100A8 in human leukemia cells, accompanied with upregulation of autophagy. RNA interference-mediated knockdown of S100A8 restored the chemosensitivity of leukemia cells, while overexpression of S100A8 enhanced drug resistance and increased autophagy. S100A8 physically interacted with the autophagy regulator BECN1 and was required for the formation of the BECN1-PI3KC3 complex. In addition, interaction between S100A8 and BECN1 relied upon the autophagic complex ULK1-mAtg13. Furthermore, we discovered that exogenous S100A8 induced autophagy, and RAGE was involved in exogenous S100A8-regulated autophagy. Our data demonstrated that S100A8 is involved in the development of chemoresistance in leukemia cells by regulating autophagy, and suggest that S100A8 may be a novel target for improving leukemia therapy. © 2014 Yang et al.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Yang, M Zeng, P Kang, R ruk9@pitt.edu RUK9 Yu, Y Yang, L Tang, D dat53@pitt.edu DAT53 Cao, L
Contributors:	Contribution Contributors Name Email Pitt Username ORCID Editor Gibson, Spencer B. UNSPECIFIED UNSPECIFIED UNSPECIFIED
Date:	12 May 2014
Date Type:	Publication
Journal or Publication Title:	PLoS ONE
Volume:	9
Number:	5
DOI or Unique Handle:	10.1371/journal.pone.0097242
Schools and Programs:	School of Medicine > Surgery
Refereed:	Yes
Date Deposited:	30 Jun 2014 15:08
Last Modified:	04 Feb 2019 15:58
URI:	http://d-scholarship.pitt.edu/id/eprint/22008

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