Single Cell Analysis of Lymph Node Tissue from HIV-1 Infected Patients Reveals that the Majority of CD4⁺ T-cells Contain One HIV-1 DNA Molecule

Josefsson, L and Palmer, S and Faria, NR and Lemey, P and Casazza, J and Ambrozak, D and Kearney, M and Shao, W and Kottilil, S and Sneller, M and Mellors, J and Coffin, JM and Maldarelli, F (2013) Single Cell Analysis of Lymph Node Tissue from HIV-1 Infected Patients Reveals that the Majority of CD4⁺ T-cells Contain One HIV-1 DNA Molecule. PLoS Pathogens, 9 (6). ISSN 1553-7366

Preview	PDF Published Version Available under License : See the attached license file. Download (569kB) | Preview
	Plain Text (licence) Available under License : See the attached license file. Download (1kB)

Abstract

Genetic recombination contributes to the diversity of human immunodeficiency virus (HIV-1). Productive HIV-1 recombination is, however, dependent on both the number of HIV-1 genomes per infected cell and the genetic relationship between these viral genomes. A detailed analysis of the number of proviruses and their genetic relationship in infected cells isolated from peripheral blood and tissue compartments is therefore important for understanding HIV-1 recombination, genetic diversity and the dynamics of HIV-1 infection. To address these issues, we used a previously developed single-cell sequencing technique to quantify and genetically characterize individual HIV-1 DNA molecules from single cells in lymph node tissue and peripheral blood. Analysis of memory and naïve CD4+ T cells from paired lymph node and peripheral blood samples from five untreated chronically infected patients revealed that the majority of these HIV-1-infected cells (>90%) contain only one copy of HIV-1 DNA, implying a limited potential for productive recombination in virus produced by these cells in these two compartments. Phylogenetic analysis revealed genetic similarity of HIV-1 DNA in memory and naïve CD4+ T-cells from lymph node, peripheral blood and HIV-1 RNA from plasma, implying exchange of virus and/or infected cells between these compartments in untreated chronic infection.

---

Share

Citation/Export:	Select format... Citation - Text Citation - HTML Endnote BibTex Dublin Core OpenURL MARC (ISO 2709) METS MODS EP3 XML Reference Manager Refer
Social Networking:	Share |

---

Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Josefsson, L Palmer, S Faria, NR Lemey, P Casazza, J Ambrozak, D Kearney, M Shao, W Kottilil, S Sneller, M Mellors, J jwm1@pitt.edu JWM1 Coffin, JM Maldarelli, F
Contributors:	Contribution Contributors Name Email Pitt Username ORCID Editor Luban, Jeremy UNSPECIFIED UNSPECIFIED UNSPECIFIED
Date:	1 June 2013
Date Type:	Publication
Journal or Publication Title:	PLoS Pathogens
Volume:	9
Number:	6
DOI or Unique Handle:	10.1371/journal.ppat.1003432
Schools and Programs:	School of Medicine > Medicine
Refereed:	Yes
ISSN:	1553-7366
Date Deposited:	01 Jul 2014 16:20
Last Modified:	23 Jun 2021 22:55
URI:	http://d-scholarship.pitt.edu/id/eprint/22125

---

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com

---

Actions (login required)

View Item