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ATPase Subdomain IA Is a Mediator of Interdomain Allostery in Hsp70 Molecular Chaperones

General, IJ and Liu, Y and Blackburn, ME and Mao, W and Gierasch, LM and Bahar, I (2014) ATPase Subdomain IA Is a Mediator of Interdomain Allostery in Hsp70 Molecular Chaperones. PLoS Computational Biology, 10 (5). ISSN 1553-734X

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The versatile functions of the heat shock protein 70 (Hsp70) family of molecular chaperones rely on allosteric interactions between their nucleotide-binding and substrate-binding domains, NBD and SBD. Understanding the mechanism of interdomain allostery is essential to rational design of Hsp70 modulators. Yet, despite significant progress in recent years, how the two Hsp70 domains regulate each other's activity remains elusive. Covariance data from experiments and computations emerged in recent years as valuable sources of information towards gaining insights into the molecular events that mediate allostery. In the present study, conservation and covariance properties derived from both sequence and structural dynamics data are integrated with results from Perturbation Response Scanning and in vivo functional assays, so as to establish the dynamical basis of interdomain signal transduction in Hsp70s. Our study highlights the critical roles of SBD residues D481 and T417 in mediating the coupled motions of the two domains, as well as that of G506 in enabling the movements of the α-helical lid with respect to the β-sandwich. It also draws attention to the distinctive role of the NBD subdomains: Subdomain IA acts as a key mediator of signal transduction between the ATP- and substrate-binding sites, this function being achieved by a cascade of interactions predominantly involving conserved residues such as V139, D148, R167 and K155. Subdomain IIA, on the other hand, is distinguished by strong coevolutionary signals (with the SBD) exhibited by a series of residues (D211, E217, L219, T383) implicated in DnaJ recognition. The occurrence of coevolving residues at the DnaJ recognition region parallels the behavior recently observed at the nucleotide-exchange-factor recognition region of subdomain IIB. These findings suggest that Hsp70 tends to adapt to co-chaperone recognition and activity via coevolving residues, whereas interdomain allostery, critical to chaperoning, is robustly enabled by conserved interactions. © 2014 General et al.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
General, IJijgenera@pitt.eduIJGENERA
Liu, Yyil43@pitt.eduYIL43
Blackburn, ME
Mao, W
Gierasch, LM
Bahar, Iivet.bahar@stonybrook.eduBAHAR
ContributionContributors NameEmailPitt UsernameORCID
Date: 1 January 2014
Date Type: Publication
Journal or Publication Title: PLoS Computational Biology
Volume: 10
Number: 5
DOI or Unique Handle: 10.1371/journal.pcbi.1003624
Schools and Programs: School of Medicine > Computational and Systems Biology
Refereed: Yes
ISSN: 1553-734X
Date Deposited: 02 Jul 2014 17:33
Last Modified: 17 Mar 2023 11:55


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