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Potent topoisomerase I inhibition by novel silatecans eliminates glioma proliferation in vitro and in vivo

Pollack, IF and Erff, M and Bom, D and Burke, TG and Strode, JT and Curran, DP (1999) Potent topoisomerase I inhibition by novel silatecans eliminates glioma proliferation in vitro and in vivo. Cancer Research, 59 (19). 4898 - 4905. ISSN 0008-5472

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Abstract

Although topoisomerase inhibitors, such as camptothecin and topotecan, have been widely used in the treatment of nonglial tumors, their application for gliomas has been limited by poor efficacy relative to toxicity that may in part reflect limited bioavailability and blood stability of these agents. However, the potential promise of this class of agents has fostered efforts to develop new, more potent, and less toxic inhibitors that may be clinically relevant. Using a cascade radical annulation route to the camptothecin family, we developed a series of novel camptothecin analogues, 7- silylcamptothecins ('silatecans'), that exhibited potent inhibition of topoisomerase I, dramatically improved blood stability, and sufficient lipophilicity to favor blood-brain barrier transit. We explored the efficacy of a series of these agents against a panel of five high-grade glioma cell lines to identify a promising compound for further preclinical testing. One of the most active agents in our systems (DB67) inhibited tumor growth in vitro with an ED50ranging between 2 and 40 ng/ml, at least 10-fold more potent than the effects observed with topotecan, and at least comparable with those of SN-38, the active metabolite of CPT-11. Because DB67 also exhibited the highest human blood stability of any of the agents examined, this agent was then selected for in vivo studies. A dose-escalation study of this agent in a nude mouse U87 glioma model system demonstrated a concentration- dependent effect, with tumor growth inhibition at day 28 postimplantation (the day control animals began to require sacrifice because of large tumor size) of 61 ± 7% and 73 ± 3% after administration of DB67 doses of 3 and 10 mg/kg/day, respectively, for 5 days beginning on postimplantation day 7. Animals that continued treatment with 10 mg/kg/day in three additional 21-day cycles all remained progression free after >90 days of follow-up but later developed enlarging tumors after treatment was stopped. However, a slightly higher dose (30 mg/kg/day) induced complete tumor regression after only two cycles in all study animals and was effective even if treatment was delayed until large, bulky tumors had developed. Application of the 30 mg/kg/day dose to treat established intracranial glioma xenografts led to long-term (>90 day) survival in six of six animals, whereas all controls died of progressive disease (P < 0.00001). No apparent toxicity was encountered in any of the treated animals. In summary, the present studies indicate that silatecans may hold significant promise for the treatment of high-grade gliomas and provide a rationale for proceeding with further preclinical evaluation of their efficacy and safety versus commercially available camptothecin derivatives.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Pollack, IFipollack@pitt.eduIPOLLACK
Erff, M
Bom, D
Burke, TG
Strode, JT
Curran, DPcurran@pitt.eduCURRAN
Date: 1 October 1999
Date Type: Publication
Journal or Publication Title: Cancer Research
Volume: 59
Number: 19
Page Range: 4898 - 4905
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0008-5472
MeSH Headings: Animals; Antineoplastic Agents, Phytogenic--therapeutic use; Antineoplastic Agents, Phytogenic--toxicity; Camptothecin--analogs & derivatives; Camptothecin--therapeutic use; Camptothecin--toxicity; Cell Division--drug effects; Cell Survival--drug effects; Genes, p53; Glioma--drug therapy; Glioma--pathology; Humans; Mice; Mice, Nude; Mutation; Structure-Activity Relationship; Topoisomerase I Inhibitors; Topotecan--therapeutic use; Topotecan--toxicity; Transplantation, Heterologous; Tumor Cells, Cultured
PubMed ID: 10519402
Date Deposited: 18 Jul 2014 20:58
Last Modified: 02 Feb 2019 15:58
URI: http://d-scholarship.pitt.edu/id/eprint/22308

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