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STUDY OF EXTRACELLULAR MATRIX SCAFFOLD REMODELING IN THE PORCINE TEMPOROMANDIBULAR JOINT: UNIAXIAL COMPRESSION AND BIOCHEMICAL ANALYSIS

Mortimer, Robert (2014) STUDY OF EXTRACELLULAR MATRIX SCAFFOLD REMODELING IN THE PORCINE TEMPOROMANDIBULAR JOINT: UNIAXIAL COMPRESSION AND BIOCHEMICAL ANALYSIS. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Regenerative medicine techniques, such as extracellular matrix (ECM) scaffolds, are currently being investigated to address temporomandibular joint (TMJ) disc replacement. Positive results were seen in the canine TMJ model, where ECM scaffolds remodeled to tissue resembling the native TMJ disc biochemically and in compressive properties (Brown et al. 2012, Brown et al. 2011). To further quantify the temporal remodeling of the ECM when implanted in the TMJ, the porcine model was chosen due to similarities with the human TMJ. These pigs underwent bilateral discectomy and a unilateral small intestine submucosa extracellular matrix (SIS-ECM) device implantation and were then euthanized at 1, 3 and 6 months post-op. Unconfined uniaxial compression was performed on the remodeled ECM, and the condylar cartilage from both joints. Biochemical characterization was also performed to measure glycosaminoglycan and DNA content. The results of this study found no statistical (p<0.a05) difference in the mechanical properties between the remodeled tissue and the native tissue at any time point. Additionally, the ipsilateral condylar cartilage was not statistically different from the native condylar cartilage except for peak stress and tangent modulus at the 30% strain rate for the 3 month post implant group. Conversely, the contralateral condylar cartilage at the 6 month time point had a statistically significant difference in the peak stress and tangent modulus at 20% and 30% strain. The mechanical findings are supported by the biochemistry, which shows no statistical difference between native and remodeled tissue. Overall, this study indicates that the SIS-ECM scaffold constructively remodels into a TMJ disc-like structure.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Mortimer, Robertrobertjmortimer90@pitt.edu
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAlmarza, Alejandroaja19@pitt.eduAJA19
Committee MemberBeniash, Eliaebeniash@pitt.eduEBENIASH
Committee MemberTaboas, Juan jmt106@pitt.eduJMT106
Date: 4 August 2014
Date Type: Publication
Defense Date: 18 July 2014
Approval Date: 4 August 2014
Submission Date: 21 July 2014
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Number of Pages: 41
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Extracellular Matrix, ECM, Temporomandibular Joint, TMJ, Compression, Biochemistry
Date Deposited: 04 Aug 2014 13:35
Last Modified: 04 Aug 2019 05:15
URI: http://d-scholarship.pitt.edu/id/eprint/22434

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