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Hypoxic conditions alter developing branchial arch-derived structures in zebrafish

Parsons, Trish E and Weinberg, Seth M and Tsang, Michael and Vieira, Alexandre R (2014) Hypoxic conditions alter developing branchial arch-derived structures in zebrafish. Dentistry 3000, 2 (1).

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Abstract

<jats:p>Background: Previous epidemiological findings have implicated hypoxia as a risk factor for craniofacial defects including cleft lip, microtia and branchial arch anomalies. This study tests the hypothesis that hypoxic exposure results in craniofacial shape variation in a zebrafish model. Methods: Three sets of zebrafish embryos were raised in uniform conditions with the exception of dissolved oxygen level.  At 24 hours past fertilization (hpf) embryos were placed in hypoxic conditions (70% or 50% dissolved oxygen tank water) and compared to unexposed control embryos.  After 24 hours of exposure to hypoxia, the embryos were incubated under normoxia.  Larvae were collected at 5 days post fertilization (dpf) and stained for cartilage. Images were taken of each specimen and subsequently landmarked to capture viscerocranial morphology.  A geometric morphometric analysis was performed to compare shape variation across groups. Results: The mean branchial arch shape of each exposure group was significantly different from controls (p&lt;0.001).  Principal components analysis revealed a clear separation of the three groups, with controls at one end of the shape spectrum, the 50% hypoxia group at the other end, and the 70% hypoxia group spanning the variation in between. Conclusions: This experiment shows that hypoxia exposure at 24hpf is capable of affecting craniofacial shape in a dose-dependent manner.  These results may have implications not only for high altitude fetal health, but other environments, behaviors and genes that affect fetal oxygen delivery. </jats:p>


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Parsons, Trish Etep13@pitt.eduTEP13
Weinberg, Seth Msmwst46@pitt.eduSMWST46
Tsang, Michaeltsang@pitt.eduTSANG
Vieira, Alexandre R
Centers: Other Centers, Institutes, Offices, or Units > Center for Craniofacial and Dental Genetics
Date: 7 August 2014
Date Type: Publication
Journal or Publication Title: Dentistry 3000
Volume: 2
Number: 1
Publisher: University Library System, University of Pittsburgh
DOI or Unique Handle: 10.5195/d3000.2014.21
Schools and Programs: School of Dental Medicine > Dental Science
Refereed: Yes
Date Deposited: 02 Sep 2014 16:48
Last Modified: 04 Aug 2020 23:58
URI: http://d-scholarship.pitt.edu/id/eprint/22874

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