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Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction

Torres-Kolbus, J and Chou, C and Liu, J and Deiters, A (2014) Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction. PLoS ONE, 9 (9).

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Abstract

Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. © 2014 Torres-Kolbus et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Torres-Kolbus, J
Chou, C
Liu, Jjil147@pitt.eduJIL147
Deiters, ADEITERS@pitt.eduDEITERS
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorSobol, Robert W.rws9@pitt.eduRWS9UNSPECIFIED
Date: 1 January 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 9
DOI or Unique Handle: 10.1371/journal.pone.0105467
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
Date Deposited: 26 Sep 2014 14:28
Last Modified: 02 Feb 2019 15:59
URI: http://d-scholarship.pitt.edu/id/eprint/23005

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