IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells

Adams, KM and Abraham, V and Spielman, D and Kolls, JK and Rubenstein, RC and Conner, GE and Cohen, NA and Kreindler, JL (2014) IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells. PLoS ONE, 9 (8).

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Abstract

The epithelium plays an active role in the response to inhaled pathogens in part by responding to signals from the immune system. Epithelial responses may include changes in chemokine expression, increased mucin production and antimicrobial peptide secretion, and changes in ion transport. We previously demonstrated that interleukin-17A (IL-17A), which is critical for lung host defense against extracellular bacteria, significantly raised airway surface pH in vitro, a finding that is common to a number of inflammatory diseases. Using microarray analysis of normal human bronchial epithelial (HBE) cells treated with IL-17A, we identified the electroneutral chloride-bicarbonate exchanger Pendrin (SLC26A4) as a potential mediator of this effect. These data were verified by real-time, quantitative PCR that demonstrated a time-dependent increase in Pendrin mRNA expression in HBE cells treated with IL-17A up to 48 h. Using immunoblotting and immunofluorescence, we confirmed that Pendrin protein expression is increased in IL-17 treated HBE cells and that it is primarily localized to the mucosal surface of the cells. Functional studies using live-cell fluorescence to measure intracellular pH demonstrated that IL-17A induced chloride-bicarbonate exchange in HBE cells that was not present in the absence of IL-17A. Furthermore, HBE cells treated with short interfering RNA against Pendrin showed substantially reduced chloride-bicarbonate exchange. These data suggest that Pendrin is part of IL-17A-dependent epithelial changes and that Pendrin may therefore be a therapeutic target in IL-17A-dependent lung disease. © 2014 Adams et al.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Adams, KM Abraham, V Spielman, D Kolls, JK jkk23@pitt.edu JKK23 Rubenstein, RC Conner, GE Cohen, NA Kreindler, JL
Contributors:	Contribution Contributors Name Email Pitt Username ORCID Editor Hartl, Dominik UNSPECIFIED UNSPECIFIED UNSPECIFIED
Date:	20 August 2014
Date Type:	Publication
Journal or Publication Title:	PLoS ONE
Volume:	9
Number:	8
DOI or Unique Handle:	10.1371/journal.pone.0103263
Schools and Programs:	School of Medicine > Immunology School of Medicine > Pediatrics
Refereed:	Yes
Date Deposited:	16 Sep 2014 19:44
Last Modified:	29 Jan 2019 15:55
URI:	http://d-scholarship.pitt.edu/id/eprint/23020

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