Seaton, KE and Ballweber, L and Lan, A and Donathan, M and Hughes, S and Vojtech, L and Moody, MA and Liao, HX and Haynes, BF and Galloway, CG and Richardson, BA and Karim, SA and Dezzutti, CS and McElrath, MJ and Tomaras, GD and Hladik, F
(2014)
HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities.
PLoS ONE, 9 (7).
Abstract
Background: Many participants in microbicide trials remain uninfected despite ongoing exposure to HIV-1. Determining the emergence and nature of mucosal HIV-specific immune responses in such women is important, since these responses may contribute to protection and could provide insight for the rational design of HIV-1 vaccines. Methods and Findings: We first conducted a pilot study to compare three sampling devices (Dacron swabs, flocked nylon swabs and Merocel sponges) for detection of HIV-1-specific IgG and IgA antibodies in vaginal secretions. IgG antibodies from HIV-1-positive women reacted broadly across the full panel of eight HIV-1 envelope (Env) antigens tested, whereas IgA antibodies only reacted to the gp41 subunit. No Env-reactive antibodies were detected in the HIV-negative women. The three sampling devices yielded equal HIV-1-specific antibody titers, as well as total IgG and IgA concentrations. We then tested vaginal Dacron swabs archived from 57 HIV seronegative women who participated in a microbicide efficacy trial in Southern Africa (HPTN 035). We detected vaginal IgA antibodies directed at HIV-1 Env gp120/gp140 in six of these women, and at gp41 in another three women, but did not detect Env-specific IgG antibodies in any women. Conclusion: Vaginal secretions of HIV-1 infected women contained IgG reactivity to a broad range of Env antigens and IgA reactivity to gp41. In contrast, Env-binding antibodies in the vaginal secretions of HIV-1 uninfected women participating in the microbicide trial were restricted to the IgA subtype and were mostly directed at HIV-1 gp120/gp140. © 2014 Seaton et al.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID ![](/images/orcid_id_24x24.png) |
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Seaton, KE | | | | Ballweber, L | | | | Lan, A | | | | Donathan, M | | | | Hughes, S | | | | Vojtech, L | | | | Moody, MA | | | | Liao, HX | | | | Haynes, BF | | | | Galloway, CG | | | | Richardson, BA | | | | Karim, SA | | | | Dezzutti, CS | csd13@pitt.edu | CSD13 | | McElrath, MJ | | | | Tomaras, GD | | | | Hladik, F | | | |
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Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID ![](/images/orcid_id_24x24.png) |
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Editor | Broliden, Kristina | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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Date: |
23 July 2014 |
Date Type: |
Publication |
Journal or Publication Title: |
PLoS ONE |
Volume: |
9 |
Number: |
7 |
DOI or Unique Handle: |
10.1371/journal.pone.0101863 |
Schools and Programs: |
School of Medicine > Obstetrics, Gynecology, and Reproductive Sciences |
Refereed: |
Yes |
Date Deposited: |
16 Sep 2014 20:07 |
Last Modified: |
26 Jan 2019 19:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/23023 |
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