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HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities

Seaton, KE and Ballweber, L and Lan, A and Donathan, M and Hughes, S and Vojtech, L and Moody, MA and Liao, HX and Haynes, BF and Galloway, CG and Richardson, BA and Karim, SA and Dezzutti, CS and McElrath, MJ and Tomaras, GD and Hladik, F (2014) HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities. PLoS ONE, 9 (7).

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Abstract

Background: Many participants in microbicide trials remain uninfected despite ongoing exposure to HIV-1. Determining the emergence and nature of mucosal HIV-specific immune responses in such women is important, since these responses may contribute to protection and could provide insight for the rational design of HIV-1 vaccines. Methods and Findings: We first conducted a pilot study to compare three sampling devices (Dacron swabs, flocked nylon swabs and Merocel sponges) for detection of HIV-1-specific IgG and IgA antibodies in vaginal secretions. IgG antibodies from HIV-1-positive women reacted broadly across the full panel of eight HIV-1 envelope (Env) antigens tested, whereas IgA antibodies only reacted to the gp41 subunit. No Env-reactive antibodies were detected in the HIV-negative women. The three sampling devices yielded equal HIV-1-specific antibody titers, as well as total IgG and IgA concentrations. We then tested vaginal Dacron swabs archived from 57 HIV seronegative women who participated in a microbicide efficacy trial in Southern Africa (HPTN 035). We detected vaginal IgA antibodies directed at HIV-1 Env gp120/gp140 in six of these women, and at gp41 in another three women, but did not detect Env-specific IgG antibodies in any women. Conclusion: Vaginal secretions of HIV-1 infected women contained IgG reactivity to a broad range of Env antigens and IgA reactivity to gp41. In contrast, Env-binding antibodies in the vaginal secretions of HIV-1 uninfected women participating in the microbicide trial were restricted to the IgA subtype and were mostly directed at HIV-1 gp120/gp140. © 2014 Seaton et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Seaton, KE
Ballweber, L
Lan, A
Donathan, M
Hughes, S
Vojtech, L
Moody, MA
Liao, HX
Haynes, BF
Galloway, CG
Richardson, BA
Karim, SA
Dezzutti, CScsd13@pitt.eduCSD13
McElrath, MJ
Tomaras, GD
Hladik, F
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorBroliden, KristinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 23 July 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 7
DOI or Unique Handle: 10.1371/journal.pone.0101863
Schools and Programs: School of Medicine > Obstetrics, Gynecology, and Reproductive Sciences
Refereed: Yes
Date Deposited: 16 Sep 2014 20:07
Last Modified: 26 Jan 2019 19:55
URI: http://d-scholarship.pitt.edu/id/eprint/23023

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