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The 43 residue DNA binding domain of gamma delta resolvase binds adjacent major and minor grooves of DNA.

Rimphanitchayakit, V and Hatfull, GF and Grindley, ND (1989) The 43 residue DNA binding domain of gamma delta resolvase binds adjacent major and minor grooves of DNA. Nucleic Acids Res, 17 (3). 1035 - 1050. ISSN 0305-1048

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Abstract

The carboxyl-terminal domain of gamma delta resolvase binds to each half of the three resolvase binding sites that constitute the recombination site, res. Ethylation inhibition experiments show that the phosphate contacts made by the C-terminal DNA binding domain are similar to those made by intact resolvase, with the exception of a single phosphate at the inside end of each contact region which is contacted solely by the intact resolvase. The DNA binding domain makes essentially identical contacts to all 6 half sites, whereas the intact resolvase makes slightly different contacts to each binding site. Despite its small size, only 43 amino acid residues, the resolvase C-terminal domain interacts with an unusually large segment of DNA. Phosphate contacts extend across an adjacent major and minor groove of DNA and about one third of the circumference around the helix. The minimal binding segment, determined experimentally, is a 12 bp sequence that includes the 9 base pair inverted repeat (common to all half sites), the adjacent 3 base pairs (towards the center of the intact resolvase binding site), and phosphates at both ends.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Rimphanitchayakit, V
Hatfull, GFgfh@pitt.eduGFH
Grindley, ND
Date: 11 February 1989
Date Type: Publication
Journal or Publication Title: Nucleic Acids Res
Volume: 17
Number: 3
Page Range: 1035 - 1050
DOI or Unique Handle: 10.1093/nar/17.3.1035
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Uncontrolled Keywords: Amino Acid Sequence, Base Sequence, Binding, Competitive, DNA, DNA-Binding Proteins, Ethylnitrosourea, Molecular Sequence Data, Nucleotide Mapping, Nucleotidyltransferases, Transposases
ISSN: 0305-1048
Funders: NIGMS NIH HHS (GM28470)
MeSH Headings: Amino Acid Sequence; Base Sequence; Binding, Competitive; DNA--metabolism; DNA-Binding Proteins--metabolism; Ethylnitrosourea; Molecular Sequence Data; Nucleotide Mapping; Nucleotidyltransferases--isolation & purification; Nucleotidyltransferases--metabolism; Transposases
Other ID: NLM PMC331720
PubMed Central ID: PMC331720
PubMed ID: 2537948
Date Deposited: 19 Nov 2014 20:21
Last Modified: 05 May 2019 02:55
URI: http://d-scholarship.pitt.edu/id/eprint/23547

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