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DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: Evidence for overlapping transcriptional units

Robinson, AC and Kenan, DJ and Hatfull, GF and Sullivan, NF and Spiegelberg, R and Donachie, WD (1984) DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: Evidence for overlapping transcriptional units. Journal of Bacteriology, 160 (2). 546 - 555. ISSN 0021-9193

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Abstract

The DNA sequence of a cloned segment of the Escherichia coli chromosome containing ftsQ, ftsA, and part of the ftsZ gene was determined and interpreted for genetic complementation and promoter fusion data for the region. The contiguous gene ftsQ, ftsA, and ftsZ were transcribed in the same direction (clockwise on the genetic map) and each had at least one associated promoter which allowed it to transcribed independently of neighboring genes. ftsA and ftsZ possessed promoters within the coding sequences of the juxtaposed upstream structural genes, and a promoter element for ftsA was surrounded by a region of twofold symmetry which corresponded closely to a symmetrical element in the region of a putative ftsZ promoter. The structural gene of ftsQ consisted of 838 nucleotides, encoding a 276-residue amino acid polypeptide of molecular weight 31,400; the structural gene of ftsA consisted of 1,260 nucleotides, encoding a 420-residue amino acid polypeptide of molecular weight 45,400. The observation that the termination codon of ftsQ overlaps with a potential initiation codon for ftsA suggested that these two genes may be translationally coupled when transcription is initiated upstream of the ftsQ coding sequence.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Robinson, AC
Kenan, DJ
Hatfull, GFgfh@pitt.eduGFH
Sullivan, NF
Spiegelberg, R
Donachie, WD
Date: 1 December 1984
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 160
Number: 2
Page Range: 546 - 555
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Amino Acid Sequence; Base Sequence; Cell Division; Cloning, Molecular; DNA Restriction Enzymes; DNA, Bacterial--genetics; Escherichia coli--cytology; Escherichia coli--genetics; Genes; Genes, Regulator; Plasmids; Promoter Regions, Genetic; Transcription, Genetic
Other ID: NLM PMC214769
PubMed Central ID: PMC214769
PubMed ID: 6094474
Date Deposited: 19 Nov 2014 20:20
Last Modified: 01 Nov 2017 14:04
URI: http://d-scholarship.pitt.edu/id/eprint/23548

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