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Gene expression in mycobacteriophage BPs

Oldfield, Lauren (2015) Gene expression in mycobacteriophage BPs. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Temperate bacteriophages can grow lytically, producing progeny phage and lysing the host, or lysogenically, integrating into the chromosome and shutting off the expression of lytic genes. The decision is controlled by a genetic switch, which senses host intracellular conditions and chooses a program of gene expression to employ. The genetic switch of mycobacteriophage BPs requires integration to produce stable repressor protein and maintain lysogeny. The regulation of this switch is controlled at the protein level, not at transcription initiation. Both integrase and repressor are tagged for proteolytic degradation and their concentrations are sensitive to host protease levels. The concentration of integrase determines the fate of BPs. The overall result of the genetic switch is determining the program of transcription. In lytic growth, genes are temporally expressed to replicate the phage genome, assemble progeny phage and lyse the host. In lysogenic growth, transcription is silenced in the phage and the repressor protein maintains repression of lytic phage genes and prevents superinfecting phage from growing lytically as well. We sought to understand the genetic switch of mycobacteriophage BPs and the transcriptional profile of gene expression. Transcription in mycobacteriophage BPs is temporally regulated and the mapping of promoter and terminator signals demonstrate additional methods of regulation in the expression and timing of transcription in mycobacteriophage BPs.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairHatfull, Graham F.gfh@pitt.eduGFH
Committee MemberArndt, Karenarndt@pitt.eduARNDT
Committee MemberBoyle, Jon P.boylej@pitt.eduBOYLEJ
Committee MemberHendrix, Roger W.rhx@pitt.eduRHX
Committee MemberKhan, Saleemkhan@pitt.eduKHAN
Date: 14 January 2015
Date Type: Publication
Defense Date: 1 May 2014
Approval Date: 14 January 2015
Submission Date: 2 December 2014
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 221
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: BPs, mycobacteriophage, transcriptome, promoter, genetic switch
Date Deposited: 14 Jan 2015 15:25
Last Modified: 15 Nov 2016 14:25


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