Fang, P and Zhou, L and Zhou, Y and Kolls, JK and Zheng, T and Zhu, Z
(2014)
Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation.
PLoS ONE, 9 (9).
Abstract
IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects of IL-22 in allergic asthma. To determine the role of IL-22 in ovalbumin-induced allergic inflammation we generated inducible lung-specific IL-22 transgenic mice. Transgenic IL-22 expression and signaling activity in the lung were determined. Ovalbumin (OVA)-induced pulmonary inflammation, immune responses, and airway hyperresponsiveness (AHR) were examined and compared between IL-22 transgenic mice and wild type controls. Following doxycycline (Dox) induction, IL-22 protein was readily detected in the large (CC10 promoter) and small (SPC promoter) airway epithelial cells. IL-22 signaling was evidenced by phosphorylated STAT3. After OVA sensitization and challenge, compared to wild type littermates, IL-22 transgenic mice showed decreased eosinophils in the bronchoalveolar lavage (BAL), and in lung tissue, decreased mucus metaplasia in the airways, and reduced AHR. Among the cytokines and chemokines examined, IL-13 levels were reduced in the BAL fluid as well as in lymphocytes from local draining lymph nodes of IL-22 transgenic mice. No effect was seen on the levels of serum total or OVA-specific IgE or IgG. These findings indicate that IL-22 has immune modulatory effects on pulmonary inflammatory responses in allergen-induced asthma.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID |
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Fang, P | | | | Zhou, L | | | | Zhou, Y | | | | Kolls, JK | jkk23@pitt.edu | JKK23 | | Zheng, T | | | | Zhu, Z | | | |
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Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID |
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Editor | Ryffel, Bernhard | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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Date: |
25 September 2014 |
Date Type: |
Publication |
Journal or Publication Title: |
PLoS ONE |
Volume: |
9 |
Number: |
9 |
DOI or Unique Handle: |
10.1371/journal.pone.0107454 |
Schools and Programs: |
School of Medicine > Pediatrics |
Refereed: |
Yes |
Other ID: |
NLM PMC4177833 |
PubMed Central ID: |
PMC4177833 |
PubMed ID: |
25254361 |
Date Deposited: |
08 May 2015 15:32 |
Last Modified: |
02 Apr 2021 21:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/24021 |
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