So, JH and Kim, JD and Yoo, KW and Kim, HT and Jung, SH and Choi, JH and Lee, MS and Jin, SW and Kim, CH
(2014)
FIH-1, a novel interactor of mindbomb, functions as an essential anti-angiogenic factor during zebrafish vascular development.
PLoS ONE, 9 (10).
Abstract
© 2014 So et al. Objective: It has been shown that Mindbomb (Mib), an E3 Ubiquitin ligase, is an essential modulator of Notch signaling during development. However, its effects on vascular development remain largely unknown.Approaches and Results: We identified a number of novel proteins that physically interact with Mib, including the Factor Inhibiting Hypoxia Inducible Factor 1 (FIH-1, also known as HIF1AN) from a yeast two hybrid screen, as previously reported. In cultured cells, FIH-1 colocalizes with Mib1, corroborating their potential interaction. In zebrafish embryos, FIH-1 appears to modulate VEGF-A signaling activity; depletion of fih-1 induces ectopic expression of vascular endothelial growth factor-a (vegfa) and leads to exuberant ectopic sprouts from intersegmental vessels (ISVs). Conversely, over-expression of fih-1 substantially attenuates the formation of ISVs, which can be rescued by concurrent over-expression of vegfa, indicating that FIH-1/HIF1AN may fine tune VEGF-A signaling.Conclusions: Taken together, our data suggest that FIH-1 interacts with Mib E3 Ubiquitin ligase and modulates vascular development by attenuating VEGF-A signaling activity.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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So, JH | juhoon@pitt.edu | JUHOON | | Kim, JD | | | | Yoo, KW | | | | Kim, HT | | | | Jung, SH | | | | Choi, JH | | | | Lee, MS | | | | Jin, SW | | | | Kim, CH | | | |
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Contributors: |
Contribution | Contributors Name | Email | Pitt Username | ORCID  |
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Editor | Ramchandran, Ramani | UNSPECIFIED | UNSPECIFIED | UNSPECIFIED |
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Date: |
27 October 2014 |
Date Type: |
Publication |
Journal or Publication Title: |
PLoS ONE |
Volume: |
9 |
Number: |
10 |
DOI or Unique Handle: |
10.1371/journal.pone.0109517 |
Schools and Programs: |
School of Medicine > Developmental Biology |
Refereed: |
Yes |
Other ID: |
NLM PMC4209967 |
PubMed Central ID: |
PMC4209967 |
PubMed ID: |
25347788 |
Date Deposited: |
12 May 2015 18:26 |
Last Modified: |
17 Oct 2019 15:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/24036 |
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