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FIH-1, a novel interactor of mindbomb, functions as an essential anti-angiogenic factor during zebrafish vascular development

So, JH and Kim, JD and Yoo, KW and Kim, HT and Jung, SH and Choi, JH and Lee, MS and Jin, SW and Kim, CH (2014) FIH-1, a novel interactor of mindbomb, functions as an essential anti-angiogenic factor during zebrafish vascular development. PLoS ONE, 9 (10).

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Abstract

© 2014 So et al. Objective: It has been shown that Mindbomb (Mib), an E3 Ubiquitin ligase, is an essential modulator of Notch signaling during development. However, its effects on vascular development remain largely unknown.Approaches and Results: We identified a number of novel proteins that physically interact with Mib, including the Factor Inhibiting Hypoxia Inducible Factor 1 (FIH-1, also known as HIF1AN) from a yeast two hybrid screen, as previously reported. In cultured cells, FIH-1 colocalizes with Mib1, corroborating their potential interaction. In zebrafish embryos, FIH-1 appears to modulate VEGF-A signaling activity; depletion of fih-1 induces ectopic expression of vascular endothelial growth factor-a (vegfa) and leads to exuberant ectopic sprouts from intersegmental vessels (ISVs). Conversely, over-expression of fih-1 substantially attenuates the formation of ISVs, which can be rescued by concurrent over-expression of vegfa, indicating that FIH-1/HIF1AN may fine tune VEGF-A signaling.Conclusions: Taken together, our data suggest that FIH-1 interacts with Mib E3 Ubiquitin ligase and modulates vascular development by attenuating VEGF-A signaling activity.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
So, JHjuhoon@pitt.eduJUHOON
Kim, JD
Yoo, KW
Kim, HT
Jung, SH
Choi, JH
Lee, MS
Jin, SW
Kim, CH
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorRamchandran, RamaniUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 27 October 2014
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 9
Number: 10
DOI or Unique Handle: 10.1371/journal.pone.0109517
Schools and Programs: School of Medicine > Developmental Biology
Refereed: Yes
Other ID: NLM PMC4209967
PubMed Central ID: PMC4209967
PubMed ID: 25347788
Date Deposited: 12 May 2015 18:26
Last Modified: 17 Oct 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/24036

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